ARG10201

anti-MCP3 antibody [113] (HRP)

anti-MCP3 antibody [113] (HRP) for ELISA and Human

Immune System antibody

Overview

Product Description HRP-conjugated Mouse Monoclonal antibody [113] recognizes Human MCP-3
Tested Reactivity Hu
Tested Application ELISA
Specificity Does not show any cross reaction with other human cytokines or growth factors tested such as IL-1β, IL-8, MCAF, SAA and EGF.
Host Mouse
Clonality Monoclonal
Clone 113
Isotype IgG1,k
Target Name MCP3
Antigen Species Human
Immunogen Recombinant human MCP-3 (M.W. 8.5kDa containing 76 amino acids residues)
Conjugation HRP
Alternate Names MCP3; NC28; Monocyte chemotactic protein 3; MARC; Small-inducible cytokine A7; FIC; C-C motif chemokine 7; Monocyte chemoattractant protein 3; MCP-3; SCYA7; SCYA6

Application Instructions

Application Note ELISA: In combination with capture antibody clone 542 (ARG10027), this HRP conjugated antibody can be used as tracer antibody for human MCP-3 detection in sandwich ELISA.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2) and 50% Glycerol
Stabilizer 50% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6354 Human CCL7

Swiss-port # P80098 Human C-C motif chemokine 7

Gene Symbol CCL7
Gene Full Name chemokine (C-C motif) ligand 7
Background Macrophage/monocyte chemotactic protein-3 (MCP-3), like other two closely related chemokines (MCP1 and MCP-2), is chemotactic to monocytes, but not to neutrophils. MCP-3 showed 78% sequence homology with MCP-1 and 58% sequence homology with MCP-2. The primary receptor for MCP-3 is D6. MCP-3 also competes with MCP-1 to bind to CCR10, and competes with Rantes and MIP-1α to bind to CCR1. MCP-3 stimulates macrophage to secrete proteases. A variety of cancer cell lines express MCP-3, suggesting a role in tumor invasion and metastasia.
Function Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. [UniProt]
Resrarch Area Immune System antibody
PTM O-glycosylated.