ARG10027

anti-MCP3 antibody [542]

anti-MCP3 antibody [542] for ELISA,Western blot and Human

Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [542] recognizes MCP3

Tested Reactivity Hu
Tested Application ELISA, WB
Specificity Does not show any cross reaction with other human cytokines or growth factors tested such as IL-1β, IL-8, MCAF, SAA and EGF.
Host Mouse
Clonality Monoclonal
Clone 542
Isotype IgG2a,k
Target Name MCP3
Antigen Species Human
Immunogen Recombinant human MCP-3 (M.W. 8.5kDa containing 76 amino acids residues)
Conjugation Un-conjugated
Alternate Names MCP3; NC28; Monocyte chemotactic protein 3; MARC; Small-inducible cytokine A7; FIC; C-C motif chemokine 7; Monocyte chemoattractant protein 3; MCP-3; SCYA7; SCYA6

Application Instructions

Application Note ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for human MCP-3 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: ARG10201). Suggested Capture Coating Dose: 3μg/well; Substrate: TMB.

Western Blot: This antibody concentration of 0.5-2μg/mL will allow visualization of 0.1 μg/lane of human MCP-3.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6354 Human CCL7

Swiss-port # P80098 Human C-C motif chemokine 7

Gene Symbol CCL7
Gene Full Name chemokine (C-C motif) ligand 7
Background This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]
Function Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. [UniProt]
Resrarch Area Immune System antibody
PTM O-glycosylated.