anti-MEK2 phospho (Thr394) antibody
anti-MEK2 phospho (Thr394) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Signaling Transduction antibody
|Product Description||Rabbit Polyclonal antibody recognizes MEK2 phospho (Thr394)|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-P, WB|
|Immunogen||Peptide sequence around phosphorylation site of threonine 394 (P-G-T(p)-P-T) derived from Human MEK-2.|
|Alternate Names||MEK 2; PRKMK2; MAPK/ERK kinase 2; EC 220.127.116.11; MAPKK2; MEK2; MAPKK 2; MAP kinase kinase 2; CFC4; MKK2; ERK activator kinase 2; Dual specificity mitogen-activated protein kinase kinase 2|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.|
|Buffer||PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||mitogen-activated protein kinase kinase 2|
|Background||Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.|
|Function||Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). [UniProt]|
|Research Area||Cancer antibody; Signaling Transduction antibody|
|Calculated MW||44 kDa|
|PTM||MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).
Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Images (3) Click the Picture to Zoom In
ARG51512 anti-MEK2 phospho (Thr394) antibody WB image
Western blot: Extracts from HepG2 and HeLa cells untreated or treated with UV stained with ARG51512 anti-MEK2 phospho (Thr394) antibody.
ARG51512 anti-MEK2 phospho (Thr394) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51512 anti-MEK2 phospho (Thr394) antibody (left) or the same antibody preincubated with blocking peptide (right).
ARG51512 anti-MEK2 phospho (Thr394) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51512 anti-MEK2 phospho (Thr394) antibody.