ARG10816

anti-MERTK phospho (Tyr749 / Tyr753 / Tyr754) antibody

anti-MERTK phospho (Tyr749 / Tyr753 / Tyr754) antibody for Confocal microscopy (Confocal),Dot blot,ELISA,ICC/IF,Immunohistochemistry,Western blot and Human,Monkey,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes MERTK phospho (Tyr749 / Tyr753 / Tyr754)
Tested Reactivity Hu, Ms, Rat, Mk
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MERTK
Species Human
Immunogen Phosphospecific peptide corresponding to aa. 746-757 of Human MERTK. (CKKI-Y(p)-SGD-Y(p)-Y(p)-RQG)
Conjugation Un-conjugated
Full Name MER proto-oncogene, tyrosine kinase
Alternate Names c-mer; Receptor tyrosine kinase MerTK; c-Eyk; Tyro12; MER; RP38; EC 2.7.10.1; Proto-oncogene c-Mer; Tyrosine-protein kinase Mer

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:250
Dot1:10000
ELISA1:10000
ICC/IF1:250
IHC1:250
WB1:750
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 110 kDa
Observed Size ~ 175 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 10461 Human MERTK

GeneID: 17289 Mouse MERTK

GeneID: 65037 Rat MERTK

Gene Symbol MERTK
Gene Full Name MER proto-oncogene, tyrosine kinase
Background This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008]
Function Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. [UniProt]
PTM Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 (By similarity).

Images (1) Click the Picture to Zoom In

  • ARG10816 anti-MERTK phospho (Tyr749 / Tyr753 / Tyr754) antibody WB image

    Western blot: 1) Phosphorylated, and 2,3) non-phosphorylated MERTK was immunoprecipitated with ARG10784 anti-MERTK antibody and stained with ARG10816 anti-MERTK phospho (Tyr749 / Tyr753 / Tyr754) antibody. MW of MERTK is 174 kDa. Lower broad band is IgG from immunoprecipitation.