anti-MGMT antibody [SQab1861]
anti-MGMT antibody [SQab1861] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot,Immunoprecipitation and Human
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab1861] recognizes MGMT|
|Tested Application||FACS, IHC-P, IP, WB|
|Immunogen||Synthetic peptide around the N-terminus of Human MGMT.|
|Alternate Names||O-6-methylguanine-DNA-alkyltransferase; Methylated-DNA--protein-cysteine methyltransferase; MGMT; EC 18.104.22.168; 6-O-methylguanine-DNA methyltransferase|
|Application Note||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||O-6-methylguanine-DNA methyltransferase|
|Background||Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. MGMT is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]|
|Function||MGMT involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProt]|
MGMT antibodies; Anti-Rabbit IgG secondary antibodies;
Cancer Pathology Markers (SQ clones)
|Calculated MW||22 kDa|
Images (4) Click the Picture to Zoom In
ARG66313 anti-MGMT antibody [SQab1861] FACS image
Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:50 dilution (blue) in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control. .
ARG66313 anti-MGMT antibody [SQab1861] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast tissue stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
ARG66313 anti-MGMT antibody [SQab1861] WB image
Western blot: 20 µg of MCF7, Jurkat, HeLa and HT-29 cell lysates stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:5000 dilution.
ARG66313 anti-MGMT antibody [SQab1861] IP image
Immunoprecipitation: 0.4 mg of HeLa whole cell lysate was immunoprecipitated (1:80 dilution) and stained with ARG66313 anti-MGMT antibody [SQab1861].
Lane 1: Immunoprecipitation in HeLa whole cell lysate
Lane 2: PBS instead of Primary Ab in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 µg (input)