ARG66313

anti-MGMT antibody [SQab1861]

anti-MGMT antibody [SQab1861] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot,Immunoprecipitation and Human

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1861] recognizes MGMT
Tested Reactivity Hu
Tested Application FACS, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1861
Isotype IgG
Target Name MGMT
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human MGMT.
Conjugation Un-conjugated
Full Name O-6-methylguanine-DNA methyltransferase
Alternate Names O-6-methylguanine-DNA-alkyltransferase; Methylated-DNA--protein-cysteine methyltransferase; MGMT; EC 2.1.1.63; 6-O-methylguanine-DNA methyltransferase

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:200
IHC-P1:100 - 1:200
IP1:80
WB1:5000 - 1:10000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 22 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 4255 Human MGMT

Swiss-port # P16455 Human Methylated-DNA--protein-cysteine methyltransferase

Gene Symbol MGMT
Gene Full Name O-6-methylguanine-DNA methyltransferase
Background Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]
Function Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProt]
Highlight Related products:
MGMT antibodies; Anti-Rabbit IgG secondary antibodies;
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Images (4) Click the Picture to Zoom In

  • ARG66313 anti-MGMT antibody [SQab1861] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:50 dilution (blue) in 1x PBS/1% BSA for 30 min at 4°C, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control. .

  • ARG66313 anti-MGMT antibody [SQab1861] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast tissue stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG66313 anti-MGMT antibody [SQab1861] WB image

    Western blot: 20 µg of MCF7, Jurkat, HeLa and HT-29 cell lysates stained with ARG66313 anti-MGMT antibody [SQab1861] at 1:5000 dilution.

  • ARG66313 anti-MGMT antibody [SQab1861] IP image

    Immunoprecipitation: 0.4 mg of HeLa whole cell lysate was immunoprecipitated (1:80 dilution) and stained with ARG66313 anti-MGMT antibody [SQab1861].

    Lane 1: Immunoprecipitation in HeLa whole cell lysate
    Lane 2: PBS instead of Primary Ab in HeLa whole cell lysate
    Lane 3: HeLa whole cell lysate, 10 µg (input)