ARG21071

anti-MHC Class I H2 Db antibody [28-14-8]

anti-MHC Class I H2 Db antibody [28-14-8] for Blocking,Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Mouse

Overview

Product Description Mouse Monoclonal antibody [28-14-8] recognizes MHC Class I H2 Db
Tested Reactivity Ms
Tested Application BL, FACS, IHC-Fr, IP
Specificity The clone 28-14-8 binds to the α3 domain of H-2Db in the presence or absence of β2 microglobulin. It cross reacts with the α3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. 28-14-8 can block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
Host Mouse
Clonality Monoclonal
Clone 28-14-8
Isotype IgG2a, kappa
Target Name MHC Class I H2 Db
Antigen Species Mouse
Immunogen C3H.SW mouse splenocytes
Conjugation Un-conjugated
Full Name histocompatibility 2, D region locus 1
Alternate Names H-2 class I histocompatibility antigen, D-B alpha chain; H2-D; B; H-2D

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 41 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14964 Mouse H2-D1

Swiss-port # P01899 Mouse H-2 class I histocompatibility antigen, D-B alpha chain

Gene Symbol H2-D1
Gene Full Name histocompatibility 2, D region locus 1
Function Involved in the presentation of foreign antigens to the immune system. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21071 anti-MHC Class I H2 Db antibody [28-14-8] FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21071 anti-MHC Class I H2 Db antibody [28-14-8] followed by ARG23802 Goat anti-Mouse IgG2a antibody (FITC) (pre-adsorbed).