ARG21080

anti-MHC Class I H2 Dd antibody [34-5-8S]

anti-MHC Class I H2 Dd antibody [34-5-8S] for Blocking,ELISA,Flow cytometry,ICC/IF,Immunoprecipitation,Purification and Mouse

Overview

Product Description Mouse Monoclonal antibody [34-5-8S] recognizes MHC Class I H2 Dd
Tested Reactivity Ms
Tested Application BL, ELISA, FACS, ICC/IF, IP, Puri
Specificity The clone 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of α1 and α2 chains when complexed with β2-microglobulin. The antibody does not react with H-2Dd α chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis.
Host Mouse
Clonality Monoclonal
Clone 34-5-8S
Isotype IgG2a, kappa
Target Name MHC Class I H2 Dd
Antigen Species Mouse
Immunogen BDF1 mouse splenocytes
Conjugation Un-conjugated
Full Name histocompatibility 2, D region locus 1
Alternate Names H-2 class I histocompatibility antigen, D-B alpha chain; H2-D; B; H-2D

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISAAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IPAssay-dependent
PuriAssay-dependent
Application Note Blocking: Antibody clone [34-5-8S] recognizing distinct D^d alpha1/alpha2 domain blocks EL4 adhesion to affinity-purified D^d. PMID: 8113669
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 41 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 14964 Mouse H2-D1

Swiss-port # P01899 Mouse H-2 class I histocompatibility antigen, D-B alpha chain

Gene Symbol H2-D1
Gene Full Name histocompatibility 2, D region locus 1
Function Involved in the presentation of foreign antigens to the immune system. [UniProt]