ARG21065

anti-MHC Class II I E kappa antibody [14-4-4S]

anti-MHC Class II I E kappa antibody [14-4-4S] for Flow cytometry,IHC-Frozen sections,ICC/IF,Immunoprecipitation,Cellular activation ,Blocking and Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [14-4-4S] recognizes MHC Class II I E kappa
Tested Reactivity Ms, Rat
Tested Application BL, Cell-Act , FACS, ICC/IF, IHC-Fr, IP
Specificity Mouse I-Ek/Rat RT1D. The clone 14-4-4S reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The antibody has been reported to cross-react with the rat MHC class II alloantigen RT1D.
Host Mouse
Clonality Monoclonal
Clone 14-4-4S
Isotype IgG2a, kappa
Target Name MHC Class II I E kappa
Species Mouse
Immunogen C3H mouse skin graft and splenocytes
Conjugation Un-conjugated
Full Name histocompatibility 2, class II antigen E alpha, pseudogene
Alternate Names AI323765; H-2Ea; MHC-H2-Ea; H2-Ea; I-Ealpha; H-2 class II histocompatibility antigen, E-U alpha chain; Ia3; E-alpha-f; Ia-3

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
Cell-Act Assay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 29 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100504404 Mouse H2-Ea-ps

Swiss-port # P14439 Mouse H-2 class II histocompatibility antigen, E-U alpha chain

Gene Symbol H2-Ea-ps
Gene Full Name histocompatibility 2, class II antigen E alpha, pseudogene
Background This locus belongs to the class II major histocompatibility complex (MHC) family of genes, which encode immune response (Ia) antigens that function in the T-cell-dependent immune response. This family member has multiple haplotypes, some of which result in the production of an E-alpha subunit that combines with an E-beta subunit to form a functional E complex at the cell surface. Other haplotypes, including that of the reference genome allele, contain mutations and they thus represent polymorphic pseudogenes that do not produce functional products. These mutations include frameshifting indels, nonsense mutations, and deletions of larger regions. The reference genome haplotype contains a deletion at the 5' end of the gene, including the core promoter region and the transcription start site, and therefore no transcripts result from this haplotype. [provided by RefSeq, Aug 2011]