anti-MICA antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
|Product Description||Rabbit Polyclonal antibody recognizes MICA|
|Tested Application||FACS, ICC/IF, IHC-P, WB|
|Immunogen||KLH-conjugated synthetic peptide corresponding to aa. 68-97 (Center) of Human MICA.|
|Full Name||MHC class I polypeptide-related sequence A|
|Alternate Names||MHC class I polypeptide-related sequence A; PERB11.1; MIC-A|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Calculated MW||43 kDa|
|Purification||Purification with Protein A and immunogen peptide.|
|Buffer||PBS and 0.09% (W/V) Sodium azide|
|Preservative||0.09% (W/V) Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||MHC class I polypeptide-related sequence A|
|Background||This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]|
|Function||Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt]|
|Cellular Localization||Cell membrane; Single- pass type I membrane protein Cytoplasm Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum|
|PTM||N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.
Images (4) Click the Picture to Zoom In
ARG55847 anti-MICA antibody ICC/IF image
Immunofluorescence: MDA-MB-231 cells stained with ARG55847 anti-MICA antibody (green). DAPI (blue) for nuclear staining.
ARG55847 anti-MICA antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG55847 anti-MICA antibody.
ARG55847 anti-MICA antibody WB image
Western blot: 20 µg of MCF7 cell lysate stained with ARG55847 anti-MICA antibody at 1:1000 dilution.
ARG55847 anti-MICA antibody FACS image
Flow Cytometry: SK-BR-3 cells stained with ARG55847 anti-MICA antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.