ARG55847

anti-MICA antibody

anti-MICA antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes MICA
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MICA
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 68-97 (Center) of Human MICA.
Conjugation Un-conjugated
Full Name MHC class I polypeptide-related sequence A
Alternate Names MHC class I polypeptide-related sequence A; PERB11.1; MIC-A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-P1:10 - 1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7
Calculated MW 43 kDa

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100507436 Human MICA

Swiss-port # Q29983 Human MHC class I polypeptide-related sequence A

Gene Symbol MICA
Gene Full Name MHC class I polypeptide-related sequence A
Background This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Function Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt]
Cellular Localization Cell membrane; Single- pass type I membrane protein Cytoplasm Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum
PTM N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.

Images (4) Click the Picture to Zoom In

  • ARG55847 anti-MICA antibody ICC/IF image

    Immunofluorescence: MDA-MB-231 cells stained with ARG55847 anti-MICA antibody (green). DAPI (blue) for nuclear staining.

  • ARG55847 anti-MICA antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG55847 anti-MICA antibody.

  • ARG55847 anti-MICA antibody WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG55847 anti-MICA antibody at 1:1000 dilution.

  • ARG55847 anti-MICA antibody FACS image

    Flow Cytometry: SK-BR-3 cells stained with ARG55847 anti-MICA antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.