ARG40184

anti-MLKL phospho (Ser358) antibody

anti-MLKL phospho (Ser358) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes MLKL phospho (Ser358)
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MLKL
Antigen Species Human
Immunogen Phosphospecific peptide around Ser358 of Human MLKL.
Conjugation Un-conjugated
Alternate Names Mixed lineage kinase domain-like protein; hMLKL

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HT-29 + TNF alpha + Smac mimetic + z-VAD
Observed Size ~ 55 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 197259 Human MLKL

Swiss-port # Q8NB16 Human Mixed lineage kinase domain-like protein

Gene Symbol MLKL
Gene Full Name mixed lineage kinase domain-like
Background This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. [UniProt]
Cellular Localization Cytoplasm. Cell membrane. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction. [UniProt]
Highlight Related products:
MLKL antibodies; MLKL Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
RIP1 activation and pathogenesis of NASH
Calculated MW 54 kDa
PTM Phosphorylation by RIPK3 induces a conformational switch that is required for necroptosis. It also induces homotrimerization and localization to the plasma membrane. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG40184 anti-MLKL phospho (Ser358) antibody WB image

    Western blot: HT-29 cells untreated (right) or treated with TNF alpha + Smac mimetic + z-VAD (left). The blots were stained with ARG40184 anti-MLKL phospho (Ser358) antibody.