ARG21506

anti-MMP1 antibody [SB12e]

anti-MMP1 antibody [SB12e] for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rabbit

nuts_pic
1
anti-MMP1 antibody [SB12e] Mouse Monoclonal antibody [SB12e] recognizes MMP1 ARG21506 Arigo biolaboratories
4.0 out of 5 based on 1
anti-MMP1 antibody [SB12e] is rated 4.0 out of 5 by 1.

anti-MMP1 antibody [SB12e]

publication_link Publication1

Overview

Product Description Mouse Monoclonal antibody [SB12e] recognizes MMP1
Tested Reactivity Hu, Rb
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Human/Rabbit MMP1.
Host Mouse
Clonality Monoclonal
Clone SB12e
Isotype IgG2b, kappa
Target Name MMP1
Antigen Species Human
Immunogen Recombinant full length MMP-1
Conjugation Un-conjugated
Full Name matrix metallopeptidase 1
Alternate Names MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA< 2 µg/ml
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100009110 Rabbit MMP1

GeneID: 4312 Human MMP1

Swiss-port # P03956 Human Interstitial collagenase

Swiss-port # P13943 Rabbit Interstitial collagenase

Gene Symbol MMP1
Gene Full Name matrix metallopeptidase 1
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
Function Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. [UniProt]
PTM Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Tyrosine phosphorylated in platelets by PKDCC/VLK.

Images (3) Click the Picture to Zoom In

  • ARG21506 anti-MMP-1 antibody [SB12e] WB image

    Western blot: 20 µg of HeLa cell lysates stained with ARG21506 anti-MMP-1 antibody [SB12e] at 1:500 dilution.

  • ARG21506 anti-MMP1 antibody [SB12e] ICC image

    Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with ARG21506 anti-MMP1 antibody [SB12e], followed by ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), DAB and mounted with Fluoromount-G.

  • ARG21506 anti-MMP1 antibody [SB12e] ICC image

    Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with ARG22307 anti-Cytokeratin 8 antibody [SB37b] and ARG21506 anti-MMP1 antibody [SB12e] followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP) (pre-adsorbed) and ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), Red AP, DAB and mounted with Fluoromount-G.

Customer's Feedback

nuts_pic      Good

Review for anti-MMP1 antibody [SB12e]

Application:WB

Sample:HUVEC

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Middermal Elastolysis: Dermal Fibroblasts Cooperate with Inflammatory Cells to the Elastolytic Disorder

IHC-P / Human

Giovanna De Cunto et al.
Mediators of Inflammation.,  (2017)

publication_link

 

hr_line