ARG20154

anti-MMP13 antibody

anti-MMP13 antibody for Western blot,Immunoprecipitation,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat,Horse,Bovine

Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
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anti-MMP13 antibody Rabbit Polyclonal antibody recognizes MMP13 ARG20154 Arigo biolaboratories
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anti-MMP13 antibody is rated 3.0 out of 5 by 1.

anti-MMP13 antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes MMP13
Tested Reactivity Hu, Ms, Rat, Bov, Hrs
Tested Application IHC-Fr, IHC-P, IP, WB
Specificity The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13. Reactivity to other species has not been tested.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MMP13
Antigen Species Rat
Immunogen Synthetic peptide surrounding amino acid 454 of rat MMP-13
Conjugation Un-conjugated
Alternate Names CLG3; EC 3.4.24.-; MANDP1; MMP-13; Collagenase 3; Matrix metalloproteinase-13

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS, 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 171052 Rat MMP13

GeneID: 17386 Mouse MMP13

GeneID: 281914 Bovine MMP13

Background The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-13 (also designated collagenase-3) is produced by breast carcinomas and degrades collagen type I, II, III.
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
PTM The proenzyme is activated by removal of the propeptide; this cleavage can be effected by other matrix metalloproteinases, such as MMP2, MMP3 and MMP14 and may involve several cleavage steps. Cleavage can also be autocatalytic, after partial maturation by another protease or after treatment with 4-aminophenylmercuric acetate (APMA) (in vitro).
N-glycosylated.
Tyrosine phosphorylated by PKDCC/VLK.

Images (1) Click the Picture to Zoom In

  • ARG20154 anti-MMP13 antibody WB image

    Western blot: Jurkat cell lysate stained with ARG20154 anti-MMP13 antibody.

    The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13.

Customer's Feedback

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Review for anti-MMP13 antibody

Application:WB

Sample:A: MDA-MB-231, B: MCF-7

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC