anti-MMP2 antibody

anti-MMP2 antibody for Western blot,ICC/IF and Human,Mouse

publication_link Publication1


Product Description Rabbit Polyclonal antibody recognizes MMP2
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MMP2
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 40-280 of Human MMP2 (NP_004521.1).
Conjugation Un-conjugated
Alternate Names CLG4A; MMP-2; TBE-1; MONA; CLG4; EC; Gelatinase A; Matrix metalloproteinase-2; MMP-II; 72 kDa gelatinase; 72 kDa type IV collagenase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NIH/3T3
Observed Size 72 kDa


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 17390 Mouse MMP2

GeneID: 4313 Human MMP2

Swiss-port # P08253 Human 72 kDa type IV collagenase

Swiss-port # P33434 Mouse 72 kDa type IV collagenase

Gene Symbol MMP2
Gene Full Name matrix metallopeptidase 2
Background This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
Function Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14.

PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.

Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. [UniProt]
Cellular Localization Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes. Isoform 2: Cytoplasm. Mitochondrion. [UniProt]
Calculated MW 74 kDa
PTM Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.

The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG40620 anti-MMP2 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG40620 anti-MMP2 antibody at 1:100 dilution.

  • ARG40620 anti-MMP2 antibody WB image

    Western blot: 25 µg of NIH/3T3 cell lysate stained with ARG40620 anti-MMP2 antibody at 1:3000 dilution.

Specific References

Hsa_circ_0091074 regulates TAZ expression via microRNA‑1297 in triple negative breast cancer cells.

Hu Jiashu et al.
International Journal of Oncology.,  (2020)