ARG23236

anti-MPO / Myeloperoxidase antibody [2C7]

anti-MPO / Myeloperoxidase antibody [2C7] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human

Inflammatory Cell Marker antibody; Neurophil Marker antibody

Overview

Product Description Mouse Monoclonal antibody [2C7] recognizes MPO / Myeloperoxidase
Tested Reactivity Hu
Species Does Not React With Rat
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 2C7
Isotype IgG1
Target Name MPO / Myeloperoxidase
Antigen Species Human
Immunogen Human myeloperoxidase.
Conjugation Un-conjugated
Alternate Names MPO; Myeloperoxidase; EC 1.11.2.2

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1:50 - 1:100
ICC/IFAssay-dependent
(Fixed with Acetone, EtOH or Methanol/acetone 70:30)
IHC-Fr1:1000 - 1:5000
IHC-P1:500 - 1:1000
WBAssay-dependent
(under non-reducing conditions)
Application Note FACS: Membrane permeabilisation is required for this application.
WB: This product recognises myeloperoxidase under non-reducing conditions.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4353 Human MPO

Swiss-port # P05164 Human Myeloperoxidase

Gene Symbol MPO
Gene Full Name myeloperoxidase
Background Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
Function Myeloperoxidase (MPO): Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. [UniProt]
Highlight Related products:
MPO antibodies; MPO ELISA Kits; MPO Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
Research Area Inflammatory Cell Marker antibody; Neurophil Marker antibody
Calculated MW 84 kDa

Images (2) Click the Picture to Zoom In

  • ARG23236 anti-MPO / Myeloperoxidase antibody [2C7] FACS image

    Flow Cytometry: Human peripheral blood granulocytes stained with ARG23236 anti-MPO / Myeloperoxidase antibody [2C7] following permeabilisation.

  • ARG23236 anti-MPO / Myeloperoxidase antibody [2C7] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin-embedded Human bone marrow tissue stained with ARG23236 anti-MPO / Myeloperoxidase antibody [2C7].