ARG67353
anti-MSK2 / RSK-B Phospho (Thr568) antibody
anti-MSK2 / RSK-B Phospho (Thr568) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MSK2 / RSK-B Phospho (Thr568) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MSK2 / RSK-B |
| Antigen Species | Human |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MSK2 / RSK-B around the phosphorylation site of Thr568. AA range:531-580 |
| Conjugation | Un-conjugated |
| Alternate Names | MSK2; Ribosomal protein S6 kinase alpha-4; S6K-alpha-4; 90 kDa ribosomal protein S6 kinase 4; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; RSKB |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 95 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol, and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol, 0.5% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O75676 Human Ribosomal protein S6 kinase alpha-4 Swiss-port # Q9Z2B9 Mouse Ribosomal protein S6 kinase alpha-4 |
|---|---|
| Gene Symbol | RPS6KA4 |
| Gene Full Name | Ribosomal protein S6 kinase alpha-4 |
| Background | This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] |
| Function | Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factor RELA, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 84 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG67353 anti-MSK2 / RSK-B Phospho (Thr568) antibody IHC-P image
Immunohistochemistry: Human brain, stained with ARG67353 anti-MSK2 / RSK-B Phospho (Thr568) antibody.
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ARG67353 anti-MSK2 / RSK-B Phospho (Thr568) antibody WB image
Western blot: 293 stained with ARG67353 anti-MSK2 / RSK-B Phospho (Thr568) antibody.
