ARG45505
anti-MSN / Moesin antibody [8D4]
anti-MSN / Moesin antibody [8D4] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Mouse Monoclonal antibody [8D4] recognizes MSN / Moesin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 8D4 |
| Isotype | IgG1 |
| Target Name | MSN / Moesin |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human MSN / Moesin. |
| Conjugation | Un-conjugated |
| Alternate Names | Moesin; Membrane-organizing extension spike protein; HEL70 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 78 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MSN |
| Gene Full Name | moesin |
| Background | Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008] |
| Function | Probably involved in connections of major cytoskeletal structures to the plasma membrane. May inhibit herpes simplex virus 1 infection at an early stage. [UniProt] |
| Cellular Localization | Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane. [UniProt] |
| Calculated MW | 68 kDa |
| PTM | Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity). Phosphorylation on Thr-558 by STK10 negatively regulates lymphocyte migration and polarization. S-nitrosylation of Cys-117 is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) implicating the iNOS-S100A8/9 transnitrosylase complex. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45505 anti-MSN / Moesin antibody [8D4] ICC/IF image
Immunofluorescence: SiHa stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 5 μg/ml dilution.
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ARG45505 anti-MSN / Moesin antibody [8D4] WB image
Western blot: HL-60 stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 0.5 μg/ml dilution.
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ARG45505 anti-MSN / Moesin antibody [8D4] FACS image
Flow Cytometry: U87 stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 1 µg/10^6 cells dilution.
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ARG45505 anti-MSN / Moesin antibody [8D4] WB image
Western blot: Rat kidney stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 0.5 μg/ml dilution.
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ARG45505 anti-MSN / Moesin antibody [8D4] IHC-P image
Immunohistochemistry: Human gastric adenocarcinoma stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 2 μg/ml dilution.
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ARG45505 anti-MSN / Moesin antibody [8D4] WB image
Western blot: COS-7 stained with ARG45505 anti-MSN / Moesin antibody [8D4] at 0.5 μg/ml dilution.
