ARG10156

anti-MUC16 / CA125 antibody [X75]

anti-MUC16 / CA125 antibody [X75] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [X75] recognizes MUC16 / CA125
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone X75
Isotype IgG1
Target Name MUC16 / CA125
Species Human
Immunogen MUC16 / CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).
Epitope Epitope specificity group B (ISOBM classification) similar to M-11.
Conjugation Un-conjugated
Full Name mucin 16, cell surface associated
Alternate Names Mucin-16; MUC-16; Ovarian carcinoma antigen CA125; CA125; Ovarian cancer-related tumor marker CA125; CA-125

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS0.1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-P1 - 5 µg/ml
WB1 - 5 µg/ml
Application Note Sandwich ELISA (Capture antibody - Detection antibody):
ARG10156 - ARG10351
ARG10351 - ARG10156
ARG10534 - ARG10156

IHC-P: Antigen Retrieval: Heat mediation was performed.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 1519 kDa

Properties

Form Liquid
Purification Ion exchange chromatography.
Buffer 10 mM Tris (pH 7.5), 0.15 M NaCl and 0.05% Sodium azide.
Preservative 0.05% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 94025 Human MUC16

Swiss-port # Q8WXI7 Human Mucin-16

Gene Symbol MUC16
Gene Full Name mucin 16, cell surface associated
Function Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. [UniProt]
Research Area Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody
PTM Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

Images (3) Click the Picture to Zoom In

  • ARG10156 anti-MUC16 / CA125 antibody [X75] IHC-P image

    Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed. The tissue section was stained with ARG10156 anti-MUC16 / CA125 antibody [X75] at 1:200 dilution.

  • ARG10156 anti-MUC16 / CA125 antibody [X75] standard curve image

    ELISA: Analysis of Human CA125 expression using ARG10156 anti-MUC16 / CA125 antibody [X75] as a capture antibody and biotinylated ARG10351 anti-MUC16 / CA125 antibody [X325] as a detection antibody with purified Human CA125 as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Plasma (green) sample at 1:2 is shown.

  • ARG10156 anti-MUC16 / CA125 antibody [X75] standard curve image

    ELISA: Analysis of Human CA125 expression using ARG10351 anti-MUC16 / CA125 antibody [X325] as a capture antibody and biotinylated ARG10156 anti-MUC16 / CA125 antibody [X75] as a detection antibody with purified Human CA125 as antigen to generate the standard curve. Detection is by HRP conjugated streptavidin and substrate. Plasma (green) sample at 1:2 is shown.

Clone References

New construction of an animal model for the orthotopic transplantation of an ovarian tumor.

IHC-P, FACS / 

Zhang H et al.
J Ovarian Res.,  (2014)

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The use of ovarian cancer cells from patients undergoing surgery to generate primary cultures capable of undergoing functional analysis.

ICC/IF / Human

O Donnell RL et al.
PLoS One.,  (2014)

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Novel treatment option for MUC16-positive malignancies with the targeted TRAIL-based fusion protein Meso-TR3.

FACS / Human

Garg G et al.
BMC Cancer.,  (2014)

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Mucin 16 is a functional selectin ligand on pancreatic cancer cells.

IP / Human

Chen SH et al.
FASEB J.,  (2012)

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