ARG45688
anti-MYPT1 antibody
anti-MYPT1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes MYPT1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | MYPT1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to N-terminal region of human MYPT1. |
| Conjugation | Un-conjugated |
| Alternate Names | PPP1R12A; protein phosphatase 1, regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; M130; MYPT1; Myosin phosphatase target subunit 1; Protein phosphatase 1 regulatory subunit 12A; Protein phosphatase myosin-binding subunit; MBS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 130 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PPP1R12A |
| Gene Full Name | protein phosphatase 1, regulatory subunit 12A |
| Background | Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
| Function | Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 115 kDa |
| PTM | Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG45688 anti-MYPT1 antibody IHC-P image
Immunohistochemistry: Human Glioma stained with ARG45688 anti-MYPT1 antibody at 2 μg/ml dilution.
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ARG45688 anti-MYPT1 antibody ICC/IF image
Immunofluorescence: U251 stained with ARG45688 anti-MYPT1 antibody at 5 μg/ml dilution.
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ARG45688 anti-MYPT1 antibody WB image
Western blot: HEK293 stained with ARG45688 anti-MYPT1 antibody at 0.5 μg/ml dilution.
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ARG45688 anti-MYPT1 antibody FACS image
Flow Cytometry: Hela stained with ARG45688 anti-MYPT1 antibody at 1 µg/10^6 cells dilution.
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ARG45688 anti-MYPT1 antibody WB image
Western blot: C6 stained with ARG45688 anti-MYPT1 antibody at 0.5 μg/ml dilution.
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ARG45688 anti-MYPT1 antibody WB image
Western blot: NIH/3T3 stained with ARG45688 anti-MYPT1 antibody at 0.5 μg/ml dilution.
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ARG45688 anti-MYPT1 antibody WB image
Western blot: COS-7 stained with ARG45688 anti-MYPT1 antibody at 0.5 μg/ml dilution.
