ARG45659
anti-Mannose Phosphate Isomerase antibody [5G5]
anti-Mannose Phosphate Isomerase antibody [5G5] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Mouse Monoclonal antibody [5G5] recognizes Mannose Phosphate Isomerase |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5G5 |
| Isotype | IgG2a |
| Target Name | Mannose Phosphate Isomerase |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human Mannose Phosphate Isomerase. |
| Conjugation | Un-conjugated |
| Alternate Names | MPI; mannose phosphate isomerase; Phosphohexomutase; EC 5.3.1.8; PMI1; CDG1B; Phosphomannose isomerase; Mannose-6-phosphate isomerase; PMI |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | MPI |
| Gene Full Name | mannose phosphate isomerase |
| Background | Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Function | Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 47 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (5) Click the Picture to Zoom In
-
ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] IHC-P image
Immunohistochemistry: Human lung cancer stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 2 μg/ml dilution.
-
ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] ICC/IF image
Immunofluorescence: T-47D stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 5 μg/ml dilution.
-
ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] WB image
Western blot: Hela stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 0.5 μg/ml dilution.
-
ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] FACS image
Flow Cytometry: U87 stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 1 µg/10^6 cells dilution.
-
ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] WB image
Western blot: Rat brain stained with ARG45659 anti-Mannose Phosphate Isomerase antibody [5G5] at 0.5 μg/ml dilution.
