ARG10737

anti-MeCP2 antibody

anti-MeCP2 antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Monkey

Overview

Product Description Rabbit Polyclonal antibody recognizes MeCP2
Tested Reactivity Hu, Ms, Rat, Mk
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MeCP2
Species Human
Immunogen Synthetic peptides from aa. 471-486 of Human MeCP2.
Conjugation Un-conjugated
Full Name methyl CpG binding protein 2
Alternate Names MRXSL; RS; MeCp2; Methyl-CpG-binding protein 2; MeCp-2 protein; RTT; AUTSX3; RTS; MRX79; PPMX; MRXS13; MRX16

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC-Fr1:1000
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 52 kDa

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 17257 Mouse MECP2

GeneID: 29386 Rat MECP2

GeneID: 4204 Human MECP2

Gene Symbol MECP2
Gene Full Name methyl CpG binding protein 2
Background DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). [UniProt]
PTM Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.

Images (5) Click the Picture to Zoom In

  • ARG10737 anti-MeCP2 antibody ICC/IF image

    Immunofluorescence: COS-1 cells stained with ARG10737 anti-MeCP2 antibody (red) at 1:5000 dilution and costained with Mouse mAb to ALDHL1 (green) at 1:1000 dilution. DAPI (blue) for nuclear staining. Following fixation with 3.7% formalin for 5 min, cells were permeabilized with ice cold methanol, blocked and stained with above antibodies.

    The MeCP2 antibody selectively stains nuclei of these African green monkey derived cells, while ALDH1L1 protein is expressed in the cytoplasm of these cells.

  • ARG10737 anti-MeCP2 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat cerebellum stained with ARG10737 anti-MeCP2 antibody (red) at 1:5000 dilution and costained with Chicken pAb to calbindin (green) at 1:2000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)

    The MeCP2 antibody selectively stains nuclei of neuronal cells to a variable degree. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.

  • ARG10737 anti-MeCP2 antibody IHC-Fr image

    Immunohistochemistry: Frozen sections of Mouse brain, which was cut at 45 µm on a vibratome after perfusion with 4% paraformaldehyde and overnight fixation, was stained with ARG10737 anti-MeCP2 antibody. MeCP2 was mainly associated with  methylated DNA in nucleus of neuron cells  (green). Blue shows DAPI staining of nuclear DNA.

  • ARG10737 anti-MeCP2 antibody WB image

    Western blot: A nuclear extract from mouse brain with ARG10737 anti-MeCP2 antibody. This antibody recognizes a strong and clear band at 75 kDa corresponding to total MeCP2  of mouse brain in SDS-PAGE, while molecular weight of MeCP2 protein is 54 kDa.

  • ARG10737 anti-MeCP2 antibody WB image

    Western blot: Rat brain (whole), Rat brain (nuclear), Mouse brain (whole), Mouse brain (nuclear), HeLa and C6 cell lysates stained with ARG10737 anti-MeCP2 antibody (green) at 1:20000 dilution.

    MeCP2 protein predominantly detected in the nuclear fraction of the lysates.