ARG10762

anti-MeCP2 phospho (Ser421) antibody [2E2]

anti-MeCP2 phospho (Ser421) antibody [2E2] for Dot blot,ICC/IF,Immunohistochemistry (PFA perfusion fixed frozen sections) and Mouse

Overview

Product Description Mouse Monoclonal antibody [2E2] recognizes MeCP2 phospho (Ser421)
Tested Reactivity Ms
Tested Application Dot, ICC/IF, IHC-FoFr
Host Mouse
Clonality Monoclonal
Clone 2E2
Isotype IgG1
Target Name MeCP2
Antigen Species Human
Immunogen KLH-conjugated synthetic phosphopeptide around Ser421 of Human MeCP2.
Conjugation Un-conjugated
Full Name methyl CpG binding protein 2
Alternate Names MRXSL; RS; MeCp2; Methyl-CpG-binding protein 2; MeCp-2 protein; RTT; AUTSX3; RTS; MRX79; PPMX; MRXS13; MRX16

Application Instructions

Application Suggestion
Tested Application Dilution
DotAssay-dependent
ICC/IF1:1000 - 1:2000
IHC-FoFr 1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 52 kDa

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 17257 Mouse MECP2

Swiss-port # Q9Z2D6 Mouse Methyl-CpG-binding protein 2

Gene Symbol MECP2
Gene Full Name methyl CpG binding protein 2
Background DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). [UniProt]
PTM Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.

Images (2) Click the Picture to Zoom In

  • ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] ICC/IF image

    Immunocytochemistry: Mouse brain section, which was cut at 45 µM on a vibratome after perfusion with 4% parformaldehyde and overnight fixation, was stained with ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] at 1:1000 dilution. Phospho-MeCP2 at S421 was mainly associated with  methylated DNA  in  nucleus of  activated neuron cells  (red). Blue shows DAPI staining of nuclear DNA. .

  • ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] Dot image

    Dot blot: 0.5 µg of Phospho-peptide or Non Phospho-peptide stained with ARG10762 anti-MeCP2 phospho (Ser421) antibody [2E2] on nitrocellulose membrane.