anti-MeCP2 phospho (Ser421) antibody
anti-MeCP2 phospho (Ser421) antibody for Western blot and Mouse
Gene Regulation antibody; Neuroscience antibody
|Product Description||Rabbit Polyclonal antibody recognizes MeCP2 phospho (Ser421)|
|Predict Reactivity||Hu, NHuPrm|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 conjugated to KLH|
|Alternate Names||MRXSL; RS; MeCp2; Methyl-CpG-binding protein 2; MeCp-2 protein; RTT; AUTSX3; RTS; MRX79; PPMX; MRXS13; MRX16|
|Application Note||Specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||methyl CpG binding protein 2|
|Background||MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser421. Phosphorylation and dephosphorylation of this site may be involved in regulation of behavioral responses to chronic antidepressant treatment (Hutchinson et al., 2012).|
|Research Area||Gene Regulation antibody; Neuroscience antibody|
|Calculated MW||52 kDa|
|PTM||Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.|
Images (1) Click the Picture to Zoom In
ARG52332 anti-MeCP2 phospho (Ser421) antibody WB image
Western blot: Mouse whole brain showing specific immunolabeling of the ~ 55 kDa truncated MeCP2 protein phosphorylated at Ser421 by using ARG52332 anti-MeCP2 phospho (Ser421) antibody.
Immunolabeling is greatly reduced by preadsorption with the phospho-peptide used as antigen (peptide).