anti-Munc18-1 phospho (Ser241) antibody
anti-Munc18-1 phospho (Ser241) antibody for Western blot and Mouse
Cancer antibody; Metabolism antibody; Neuroscience antibody
|Product Description||Rabbit Polyclonal antibody recognizes Munc18-1 phospho (Ser241)|
|Predict Reactivity||Hu, Rat, Bov, Chk, Dog, NHuPrm, Sheep|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser241 conjugated to KLH|
|Alternate Names||p67; Unc18-1; Protein unc-18 homolog A; MUNC18-1; RBSEC1; NSEC1; Protein unc-18 homolog 1; UNC18; Unc-18A; P67; Syntaxin-binding protein 1; N-Sec1|
|Application Note||Specific for the ~65k Munc18-1 protein phosphorylated at Ser241. Immunolabeling is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||syntaxin binding protein 1|
|Background||Munc-18 (mammalian homologue of Unc-18) is a protein that is thought to be involved in regulating exocytosis due, at least in part, to it ability to bind syntaxin (Ciufo et al., 2005). Munc18-1 is a neuron-specific member of the Sec1/Munc18 protein family that binds to syntaxin1A and is thought to stabilize the complex (Liu et al., 2004). The function of Munc18-1 is thought to be regulated by phosphorylation.|
|Research Area||Cancer antibody; Metabolism antibody; Neuroscience antibody|
|Calculated MW||68 kDa|
Images (1) Click the Picture to Zoom In
ARG52342 anti-Munc18-1 phospho (Ser241) antibody WB image
Western blot: Mouse hippocampus lysate showing specific immunolabeling of the ~65k Munc18-1 protein phosphorylated at Ser 241 stained with ARG52342 anti-Munc18-1 phospho (Ser241) antibody.
Phosphospecificity is shown in the second lane where immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase).