anti-Myc phospho (Thr58) antibody
anti-Myc phospho (Thr58) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
|Product Description||Rabbit Polyclonal antibody recognizes Myc phospho (Thr58)|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||IHC-P, WB|
|Immunogen||Peptide sequence around phosphorylation site of threonine 58 (L-P-T(p)-P-P) derived from Human Myc.|
|Alternate Names||c-Myc; MRTL; MYCC; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; bHLHe39; Myc proto-oncogene protein; Transcription factor p64|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.|
|Buffer||PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||v-myc avian myelocytomatosis viral oncogene homolog|
|Background||Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.|
|Function||Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]|
|Highlight||Related Antibody Duos and Panels:
ARG30012 Phospho Myc Antibody Duo (pS62, pS58)
ARG30124 Phospho c-Myc Antibody Duo (Total, pT58)
ARG30126 Phospho c-Myc Antibody Panel (Total, pT58, pS62)
Myc antibodies; Myc ELISA Kits; Myc Duos / Panels; Anti-Rabbit IgG secondary antibodies;
|Research Area||Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody|
|Calculated MW||49 kDa|
|PTM||Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).
Images (2) Click the Picture to Zoom In
ARG51538 anti-Myc phospho (Thr58) antibody WB image
Western blot: Extracts from HeLa cells stained with ARG51538 anti-Myc phospho (Thr58) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
ARG51538 anti-Myc phospho (Thr58) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51538 anti-Myc phospho (Thr58) antibody (left) or the same antibody preincubated with blocking peptide (right).