anti-Myc tag antibody (Agarose)
anti-Myc tag antibody (Agarose) for Immunoprecipitation and Other
Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Agarose-conjugated Goat Polyclonal antibody recognizes Myc tag
|Target Name||Myc tag|
|Immunogen||c-myc (EKLISEEDL) coupled to KLH. Antibody coupled to agarose beads using a cyanogen bromide method.|
|Alternate Names||c-Myc; MRTL; MYCC; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; bHLHe39; Myc proto-oncogene protein; Transcription factor p64|
|Application Note||Immunoprecipitation: 15 to 25 ul of gell slurry per 0.1 to 1 mg of protein lysate or extract
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||0.01M Phosphate (pH 7.2), 0.1M NaCl and 0.1% Sodium azide|
|Preservative||0.1% Sodium azide|
|Storage Instruction||Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||v-myc avian myelocytomatosis viral oncogene homolog|
|Background||The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]|
|Function||Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. [UniProt]|
|Research Area||Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody|
|Calculated MW||49 kDa|
|PTM||Phosphorylated by PRKDC. Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence. Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).