ARG20574

anti-MyoD antibody [5.8A]

anti-MyoD antibody [5.8A] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation and Chicken,Human,Mouse,Rat

Developmental Biology antibody; Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [5.8A] recognizes MyoD
Tested Reactivity Hu, Ms, Rat, Chk
Tested Application ICC/IF, IHC-Fr, IHC-P, IP
Host Mouse
Clonality Monoclonal
Clone 5.8A
Isotype IgG1
Target Name MyoD
Species Mouse
Immunogen Recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
Conjugation Un-conjugated
Full Name myogenic differentiation 1
Alternate Names MYF3; Myoblast determination protein 1; bHLHc1; Myod-1; AI503393; MyoD; PUM; Myogenic factor 3; MYOD; Myf-3; Class C basic helix-loop-helix protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-Fr2 - 4 µg/ml
IHC-P2 - 4 µg/ml
IP2 µg/mg
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rhabdomyosarcoma, SW80 cells
Calculated MW 35 kDa

Properties

Form Liquid
Purification Purification with Protein A/G.
Buffer PBS and 0.08% Sodium azide
Preservative 0.08% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 17927 Mouse MYOD1

GeneID: 337868 Rat MYOD1

GeneID: 4654 Human MYOD1

Gene Symbol Myod1
Gene Full Name myogenic differentiation 1
Background This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. [UniProt]
Research Area Developmental Biology antibody; Gene Regulation antibody
PTM Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.