ARG51580

anti-MyoD phospho (Ser200) antibody

anti-MyoD phospho (Ser200) antibody for Western blot and Human,Mouse,Rat

Developmental Biology antibody; Gene Regulation antibody
publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes MyoD phospho (Ser200)
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name MyoD
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD.
Conjugation Un-conjugated
Alternate Names PUM; MYF3; Myoblast determination protein 1; bHLHc1; Myogenic factor 3; MYOD; Myf-3; Class C basic helix-loop-helix protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17927 Mouse MYOD1

GeneID: 337868 Rat MYOD1

GeneID: 4654 Human MYOD1

Gene Symbol MYOD1
Gene Full Name myogenic differentiation 1
Background MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins (By similarity). [UniProt]
Research Area Developmental Biology antibody; Gene Regulation antibody
Calculated MW 35 kDa
PTM Phosphorylated by CDK9. This phosphorylation promotes its function in muscle differentiation.
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function (By similarity).
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
Methylation at Lys-104 by EHMT2/G9a inhibits myogenic activity.

Images (1) Click the Picture to Zoom In

  • ARG51580 anti-MyoD phospho (Ser200) antibody WB image

    Western blot: Extracts from HeLa cells untreated or treated with EGF stained with ARG51580 anti-MyoD phospho (Ser200) antibody.

Specific References

Discoidin Domain Receptor 1 Regulates Runx2 during Osteogenesis of Osteoblasts and Promotes Bone Ossification via Phosphorylation of p38.

WB / Mouse

Liang-Yin Chou et al.
Int J Mol Sci.,  (2020)

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