ARG22587

anti-N Cadherin antibody [13A9]

anti-N Cadherin antibody [13A9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

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anti-N Cadherin antibody [13A9]

Mouse Monoclonal antibody [13A9] recognizes Cadherin-N/N-Cadherin
This antibody recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry.N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. Mouse anti Human N-cadherin antibody, clone 13A9 studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999).Mouse anti Human N-cadherin antibody, clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).

ARG22587 Arigo biolaboratories
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anti-N Cadherin antibody [13A9]

Overview

Product Description

Mouse Monoclonal antibody [13A9] recognizes Cadherin-N/N-Cadherin
This antibody recognizes neural cadherin, otherwise known as CD325, a calcium dependent cell-cell adhesion glycoprotein, and member of the cadherin superfamily, which links to the actin cytoskeleton via catenins, and plays a role in cell-matrix adhesion, cell growth and differentiation, and the establishment of left-right asymmetry.N-cadherin is expressed by neurons, endothelial cells, muscle cells, and stem cells, and is one of the primary cadherins recruited to the site of neuronal synapse formation. N-cadherin is directly involved in the differentiation of early hematopoietic progenitors, and is commonly expressed by cancer cells, playing a role in transendothelial migration and metastasis, through the up-regulation of the src kinase pathway, and subsequent failure of the intercellular connection between two adjacent endothelial cells. Mouse anti Human N-cadherin antibody, clone 13A9 studies have demonstrated that expression levels of E-Cadherin and N-Cadherin have a role to play in the invasive properties of breast cancer. Decreased levels of E-cadherin and loss of E-cadherin-mediated adhesion, can result in the transition of a benign epithelial tumor to an invasive tumor, and increase invasiveness, whilst the expression of N-cadherin correlates with motility, invasiveness and tumor metastasis, irrespective of the presence of E-cadherin (Nieman et al. 1999).Mouse anti Human N-cadherin antibody, clone 13A9 has been shown to be specific for N-cadherin, and does not recognize E-cadherin, M-cadherin or P-cadherin (Knudsen et al. 1995). Immunohistological studies have shown that clone 13A9 can be used as a reliable marker for the differential diagnosis of pleural mesotheliomas and lung adenocarcinomas, when used in conjunction with E-cadherin (Han et al. 1997).

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone 13A9
Isotype IgG1
Target Name N Cadherin
Antigen Species Human
Immunogen Recombinant MBP fusion protein containing the entire cytoplasmic domain of human N-cadherin.
Conjugation Un-conjugated
Full Name cadherin 2
Alternate Names Neural cadherin; N-cadherin; CD antigen CD325; Cadherin-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50-1:200
IHC-Fr1:50-1:200
IHC-P1:50-1:200
IP2 µg
WB1:100 - 1:1000
Application Note IHC-P: This product requires antigen retrieval using steam heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: MCA5698 detects a band of approximately 135-140kDa in human HT-1080 and HeLa cell lysates.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1000 Human CDH2

GeneID: 12558 Mouse CDH2

GeneID: 83501 Rat CDH2

Gene Symbol Cdh2
Gene Full Name cadherin 2
Background cell-cell adhesion molecule; may play a role in smooth muscle cell migration and adhesion in response to injury [RGD, Feb 2006]
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). [UniProt]
PTM Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).
May be phosphorylated by OBSCN.

Images (3) Click the Picture to Zoom In

  • ARG22587 anti-N Cadherin antibody [13A9] WB image

    Western blot: 20 µg of Mouse brain lysate stained with ARG22587 anti-N Cadherin antibody [13A9] at 1:1000 dilution.

  • ARG22587 anti-Cadherin-N/N-Cadherin antibody [13A9] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer biopsy stained with ARG22587 anti-Cadherin-N/N-Cadherin antibody [13A9] followed by HRP polymer detection and DAB substrate development. Antigen Retrieval: Boil tissue section in citrate buffer (pH 6.2). (low power).

  • ARG22587 anti-Cadherin-N/N-Cadherin antibody [13A9] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer biopsy stained with ARG22587 anti-Cadherin-N/N-Cadherin antibody [13A9] followed by HRP polymer detection and DAB substrate development. Antigen Retrieval: Boil tissue section in citrate buffer (pH 6.2). (high power).

Customer's Feedback

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Review for anti-N Cadherin antibody [13A9]

Application:WB

Sample:Mouse brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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