ARG57525

anti-N Cadherin antibody [8C11]

anti-N Cadherin antibody [8C11] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

EMT Study antibody; Mesenchymal Markers antibody

Overview

Product Description Mouse Monoclonal antibody [8C11] recognizes N Cadherin
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 8C11
Isotype IgG1, kappa
Target Name N Cadherin
Antigen Species Human
Immunogen Partial recombinant protein of Human N-Cadherin (extracellular domain).
Conjugation Un-conjugated
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Alternate Names Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P0.5 - 2 µg/ml
WB0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM Tris with 1mM EDTA (pH 9.0) for 10-20 min followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW ~ 100 kDa (unmodified), 125-140 kDa (modified).

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1000 Human CDH2

GeneID: 12558 Mouse CDH2

GeneID: 83501 Rat CDH2

Gene Symbol CDH2
Gene Full Name cadherin 2, type 1, N-cadherin (neuronal)
Background N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
Function N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein
Cell membrane; sarcolemma
Cell junction
Cell surface
Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. [UniProt]
Research Area EMT Study antibody; Mesenchymal Markers antibody
PTM Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).

May be phosphorylated by OBSCN. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG57525 anti-N Cadherin antibody [8C11] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse heart tissue stained with ARG57525 anti-N Cadherin antibody [8C11]. Antigen Retrieval: Boil tissue section in 10mM Tris with 1mM EDTA (pH 9.0) for 10-20 min followed by cooling at RT for 20 min.

  • ARG57525 anti-N Cadherin antibody [8C11] WB image

    Western blot: Human brain lysate stained with ARG57525 anti-N Cadherin antibody [8C11].