anti-N Cadherin antibody [8C11]
anti-N Cadherin antibody [8C11] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
|Product Description||Mouse Monoclonal antibody [8C11] recognizes N Cadherin|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||FACS, ICC/IF, IHC-P, WB|
|Target Name||N Cadherin|
|Immunogen||Partial recombinant protein of Human N-Cadherin (extracellular domain).|
|Full Name||cadherin 2, type 1, N-cadherin (neuronal)|
|Alternate Names||Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in 10mM Tris with 1mM EDTA (pH 9.0) for 10-20 min followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Calculated MW||~ 100 kDa (unmodified), 125-140 kDa (modified).|
|Purification||Purification with Protein G.|
|Buffer||PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.|
|Preservative||0.05% Sodium azide|
|Stabilizer||0.1 mg/ml BSA|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||cadherin 2, type 1, N-cadherin (neuronal)|
|Background||This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq, Jul 2008]|
|Function||Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). [UniProt]|
|Cellular Localization||Cell membrane; Single-pass type I membrane protein
Cell membrane; sarcolemma
Note: Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. [UniProt]
|PTM||Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).
May be phosphorylated by OBSCN. [UniProt]
Images (2) Click the Picture to Zoom In
ARG57525 anti-N Cadherin antibody [8C11] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse heart tissue stained with ARG57525 anti-N Cadherin antibody [8C11]. Antigen Retrieval: Boil tissue section in 10mM Tris with 1mM EDTA (pH 9.0) for 10-20 min followed by cooling at RT for 20 min.
ARG57525 anti-N Cadherin antibody [8C11] WB image
Western blot: Human brain lysate stained with ARG57525 anti-N Cadherin antibody [8C11].