anti-N Cadherin antibody

anti-N Cadherin antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Sheep

EMT Study antibody; Mesenchymal Markers antibody
publication_link Publication2


Product Description Rabbit Polyclonal antibody recognizes N Cadherin
Tested Reactivity Hu, Ms, Rat, Sheep
Predict Reactivity Chimp, Chk
Tested Application ELISA, FACS, ICC/IF, IHC-P, IP, WB
Specificity The antibody detects a band at ~ 130 kDa in western blots of Human endothelial cells and Mouse brain tissue, and does not cross-react with E Cadherin.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name N Cadherin
Antigen Species Human
Immunogen Carrier protein coupled synthetic peptide corresponding to aa. 811-824 of Human N Cadherin.
Conjugation Un-conjugated
Alternate Names Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 µg/ml
Application Note WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 130 kDa


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.
Preservative 0.05% Sodium azide.
Stabilizer 50% Glycerol and 1 mg/ml BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 1000 Human CDH2

GeneID: 12558 Mouse CDH2

GeneID: 83501 Rat CDH2

Gene Symbol CDH2
Gene Full Name cadherin 2, type 1, N-cadherin (neuronal)
Background N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
Function N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Cell membrane, sarcolemma. Cell junction. Cell surface. Note=Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30320 EMT Marker Antibody Panel
Related products:
N Cadherin antibodies; N Cadherin ELISA Kits; N Cadherin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
New EMT antibody panel is released
Research Area EMT Study antibody; Mesenchymal Markers antibody
Calculated MW 100 kDa
PTM Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).

May be phosphorylated by OBSCN. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG23870 anti-N Cadherin antibody WB image

    Western blot: 20 µg of mouse brain stained with ARG23870 anti-N Cadherin antibody at 1:1000 dilution. (Observed MW: ~130 kDa)

  • ARG23870 anti-N Cadherin antibody ICC/IF image

    Immunofluorescence: MCF7-ADR cells stained with ARG23870 anti-N Cadherin antibody.

  • ARG23870 anti-N Cadherin antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded human pancreatic adenocarcinoma stained with ARG23870 anti-N Cadherin antibody.

  • ARG23870 anti-N Cadherin antibody WB image

    Western blot: Human endothelial cells untreated (left) or treated with Pervanadate (1 mM) for 30 min (right). The blots were stained with ARG23870 anti-N Cadherin antibody. (Observed MW: ~130 kDa)

  • ARG23870 anti-N Cadherin antibody IP image

    Immunoprecipitation: Mouse brain lysate immunoprecipitated with no antibody (lane 1), ARG23870 anti-N Cadherin antibody (lane 2) and whole mouse brain lysate (lane 3). The blot was stained with anti-N Cadherin (Cytoplasmic) Mouse monoclonal antibody (lanes 1-3).

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Sample:Mouse Brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

OC-STAMP Overexpression Drives Lung Alveolar Epithelial Cell Type II Senescence in Silicosis

WB / Rat

Tian Li et al.
Oxid Med Cell Longev.,  (2021)




Thalidomide Attenuates Skin Lesions and Inflammation in Rosacea-Like Mice Induced by Long-Term Exposure of LL-37

WB / Mouse

Yumeng Kang et al.
Drug Design, Development and Therapy,  (2022)