anti-N Cadherin antibody [SQab22260]

anti-N Cadherin antibody [SQab22260] for IHC-Formalin-fixed paraffin-embedded sections and Human

EMT Study antibody; Mesenchymal Markers antibody


Product Description Recombinant Rabbit Monoclonal antibody [SQab22260] recognizes N Cadherin
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab22260
Isotype IgG
Target Name N Cadherin
Antigen Species Human
Immunogen Synthetic peptide corresponding to residues within C-Terminal of N-Cadherin.
Conjugation Un-conjugated
Protein Full Name Cadherin-2
Alternate Names Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human liver tissue


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 1000 Human CDH2

Swiss-port # P19022 Human Cadherin-2

Gene Symbol CDH2
Gene Full Name cadherin 2, type 1, N-cadherin (neuronal)
Background N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
Function N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]
Cellular Localization Cell membrane, Single-pass type I membrane protein. [UniProt]
Research Area EMT Study antibody; Mesenchymal Markers antibody
Calculated MW 100 kDa
PTM Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).

May be phosphorylated by OBSCN. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG66921 anti-N Cadherin antibody [SQab22260] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human liver tissue stained with ARG66921 anti-N Cadherin antibody [SQab22260]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).