ARG59259
anti-NCKAP1 antibody
anti-NCKAP1 antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes NCKAP1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | NCKAP1 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-250 of Human NCKAP1 (NP_038464.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Membrane-associated protein HEM-2; NAP1; NAP 1; NAP125; p125Nap1; Nck-associated protein 1; HEM2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | MCF7 | ||||
| Observed Size | 125 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NCKAP1 |
| Gene Full Name | NCK-associated protein 1 |
| Function | Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. [UniProt] |
| Cellular Localization | Cell membrane; Single-pass membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side. Note=At the interface between the lamellipodial actin meshwork and the membrane. [UniProt] |
| Calculated MW | 129 kDa |
Images (1) Click the Picture to Zoom In
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ARG59259 anti-NCKAP1 antibody WB image
Western blot: 25 µg of MCF7 cell lysate stained with ARG59259 anti-NCKAP1 antibody at 1:3000 dilution.
