ARG10666

anti-NDUFA1 antibody

anti-NDUFA1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFA1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFA1
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Human NDUFA1. (ISGVDRYYVSKGLENID)
Conjugation Un-conjugated
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
Alternate Names MWFE; ZNF183; NADH-ubiquinone oxidoreductase MWFE subunit; CI-MWFE; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; Complex I-MWFE

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF0.5 - 1 µg/ml
IHC-Fr0.5 - 1 µg/ml
IHC-P0.5 - 1 µg/ml
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 4694 Human NDUFA1

GeneID: 54405 Mouse NDUFA1

Swiss-port # O15239 Human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Swiss-port # O35683 Mouse NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Gene Symbol NDUFA1
Gene Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
Background The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008]
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG10666 anti-NDUFA1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat heart tissue stained with ARG10666 anti-NDUFA1 antibody.

  • ARG10666 anti-NDUFA1 antibody WB image

    Western blot: 1) Rat heart, 2) Rat liver, 3) Rat kidney, 4) Rat brain, and 5) Human HT1080 cell lysates stained with ARG10666 anti-NDUFA1 antibody.

  • ARG10666 anti-NDUFA1 antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat heart tissue stained with ARG10666 anti-NDUFA1 antibody.