anti-NDUFA1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,ICC/IF and Human,Mouse,Rat
|Product Description||Rabbit Polyclonal antibody recognizes NDUFA1|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-Fr, IHC-P, WB|
|Immunogen||Synthetic peptide around the C-terminus of Human NDUFA1. (ISGVDRYYVSKGLENID)|
|Alternate Names||MWFE; ZNF183; NADH-ubiquinone oxidoreductase MWFE subunit; CI-MWFE; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; Complex I-MWFE|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Affinity purification with immunogen.|
|Buffer||PBS, 0.025% Sodium azide and 2.5% BSA.|
|Preservative||0.025% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa|
|Background||The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008]|
|Function||Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [UniProt]|
|Calculated MW||8 kDa|
Images (3) Click the Picture to Zoom In
ARG10666 anti-NDUFA1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat heart tissue stained with ARG10666 anti-NDUFA1 antibody.
ARG10666 anti-NDUFA1 antibody WB image
Western blot: 1) Rat heart, 2) Rat liver, 3) Rat kidney, 4) Rat brain, and 5) Human HT1080 cell lysates stained with ARG10666 anti-NDUFA1 antibody.
ARG10666 anti-NDUFA1 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat heart tissue stained with ARG10666 anti-NDUFA1 antibody.