ARG45819

anti-NDUFA9 antibody

anti-NDUFA9 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFA9
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFA9
Antigen Species Human
Immunogen Recombinant protein containing to human NDUFA9.
Conjugation Un-conjugated
Alternate Names NDUFA9; NADH:Ubiquinone Oxidoreductase Subunit A9; SDR22E1; NDUFS2L; CI-39k; COQ11; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 9, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 9, 39kDa; Short Chain Dehydrogenase/Reductase Family 22E, Member 1; NADH-Ubiquinone Oxidoreductase 39 KDa Subunit; Complex I 39kDa Subunit; CI-39kD; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 2-Like (NADH-Coenzyme Q Reductase); NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 9 (39kD); Complex I-39kD; MC1DN26; CI39k; CC6

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 36 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 362440 Rat NDUFA9

GeneID: 4704 Human NDUFA9

GeneID: 66108 Mouse NDUFA9

Gene Symbol NDUFA9
Gene Full Name NADH:Ubiquinone Oxidoreductase Subunit A9
Background The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. A pseudogene has been identified on chromosome 12. [provided by RefSeq, May 2010]
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Required for proper complex I assembly. [UniProt]
Cellular Localization Mitochondrion. [UniProt]
Calculated MW 43 kDa
PTM Acetylation. [UniProt]

Images (6) Click the Picture to Zoom In

  • ARG45819 anti-NDUFA9 antibody IHC-P image

    Immunohistochemistry: Human liver cancer stained with ARG45819 anti-NDUFA9 antibody at 2 μg/ml dilution.

  • ARG45819 anti-NDUFA9 antibody WB image

    Western blot: CACO-2 stained with ARG45819 anti-NDUFA9 antibody at 0.5 μg/ml dilution.

  • ARG45819 anti-NDUFA9 antibody FACS image

    Flow Cytometry: U87 stained with ARG45819 anti-NDUFA9 antibody at 1 µg/10^6 cells dilution.

  • ARG45819 anti-NDUFA9 antibody IHC-P image

    Immunohistochemistry: Rat heart stained with ARG45819 anti-NDUFA9 antibody at 2 μg/ml dilution.

  • ARG45819 anti-NDUFA9 antibody WB image

    Western blot: RH35 stained with ARG45819 anti-NDUFA9 antibody at 0.5 μg/ml dilution.

  • ARG45819 anti-NDUFA9 antibody WB image

    Western blot: HEPA1-6 stained with ARG45819 anti-NDUFA9 antibody at 0.5 μg/ml dilution.