ARG45712

anti-NDUFS5 antibody

anti-NDUFS5 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NDUFS5
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NDUFS5
Antigen Species Human
Immunogen Recombinant protein containing to human NDUFS5.
Conjugation Un-conjugated
Alternate Names NDUFS5; NADH:Ubiquinone Oxidoreductase Subunit S5; NADH Dehydrogenase [Ubiquinone] Iron-Sulfur Protein 5; CI-15k; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 5, 15kDa (NADH-Coenzyme Q Reductase); Complex I-15 KDa; CI-15 KDa; NADH Dehydrogenase (Ubiquinone) Fe-S Protein 5 (15kD) (NADH-Coenzyme Q Reductase); NADH:Ubiquinone Oxidoreductase 15 KDa IP Subunit; NADH-Ubiquinone Oxidoreductase 15 KDa Subunit; Complex I 51kDa Subunit; CI15K

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 15 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4725 Human NDUFS5

GeneID: 595136 Mouse NDUFS5

Swiss-port # O43920 Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Swiss-port # Q99LY9 Mouse NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Gene Symbol NDUFS5
Gene Full Name NADH:Ubiquinone Oxidoreductase Subunit S5
Background This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17. [provided by RefSeq, May 2010]
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. . [UniProt]
Cellular Localization Membrane; Mitochondrion; Mitochondrion inner membrane. [UniProt]
Calculated MW 13 kDa
PTM Disulfide bond. [UniProt]

Images (7) Click the Picture to Zoom In

  • ARG45712 anti-NDUFS5 antibody IHC-P image

    Immunohistochemistry: Human liver cancer stained with ARG45712 anti-NDUFS5 antibody at 2 μg/ml dilution.

  • ARG45712 anti-NDUFS5 antibody WB image

    Western blot: K562 stained with ARG45712 anti-NDUFS5 antibody at 0.5 μg/ml dilution.

  • ARG45712 anti-NDUFS5 antibody FACS image

    Flow Cytometry: Hela stained with ARG45712 anti-NDUFS5 antibody at 1 µg/10^6 cells dilution.

  • ARG45712 anti-NDUFS5 antibody IHC-P image

    Immunohistochemistry: Rat heart stained with ARG45712 anti-NDUFS5 antibody at 2 μg/ml dilution.

  • ARG45712 anti-NDUFS5 antibody WB image

    Western blot: Rat liver stained with ARG45712 anti-NDUFS5 antibody at 0.5 μg/ml dilution.

  • ARG45712 anti-NDUFS5 antibody IHC-P image

    Immunohistochemistry: Mouse heart stained with ARG45712 anti-NDUFS5 antibody at 2 μg/ml dilution.

  • ARG45712 anti-NDUFS5 antibody WB image

    Western blot: Mouse liver stained with ARG45712 anti-NDUFS5 antibody at 0.5 μg/ml dilution.