ARG20457

anti-NIK antibody

anti-NIK antibody for Western blot and Human,Mouse,Rat,Dog,Hamster

Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes NIK
Tested Reactivity Hu, Ms, Rat, Dog, Hm
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NIK
Species Human
Immunogen Synthetic peptide around 927 aa of Human NIK protein (NP_003945.2)
Conjugation Un-conjugated
Full Name mitogen-activated protein kinase kinase kinase 14
Alternate Names NIK; HSNIK; FTDCR1B; HS; EC 2.7.11.25; HsNIK; Serine/threonine-protein kinase NIK; NF-kappa-beta-inducing kinase; Mitogen-activated protein kinase kinase kinase 14

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 104 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2), 50% Glycerol, 1% BSA and 0.02% Thimerosal
Preservative 0.02% Thimerosal
Stabilizer 50% Glycerol, 1% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 53859 Mouse MAP3K14

GeneID: 9020 Human MAP3K14

Swiss-port # Q99558 Human Mitogen-activated protein kinase kinase kinase 14

Swiss-port # Q9WUL6 Mouse Mitogen-activated protein kinase kinase kinase 14

Gene Symbol MAP3K14
Gene Full Name mitogen-activated protein kinase kinase kinase 14
Background NIK (NF-kB-inducing kinase) is a member of the MAP kinase kinase kinase family that binds TRAF2 and stimulates NF-kB activity. NIK was initially isolated from a human B cell cDNA library and contains 795 amino acids with an apparent molecular weight of slightly more than 97 kDa on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation.
Highlight Related products:
NIK antibodies; Anti-Rabbit IgG secondary antibodies;
Related poster download:
The NF-kappa B Pathways.pdf
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
PTM Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization.
Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it.

Images (2) Click the Picture to Zoom In

  • ARG20457 anti-NIK antibody WB image

    Western blot: 30 μg of HeLa cell with no treatment (CTRL) or treat with 5 ng/ml TNF-a for 5 min, 10 min, and 20 min, respectively (5, 10, 20). The blots were stained with ARG20457 anti-NIK antibody at 1:500 dilution.

  • ARG20457 anti-NIK antibody WB image

    Western Blot: 1, 2) Jurkat cell lysates stained with ARG20457 anti-NIK antibody.