ARG52366

anti-NMDA NR2B Subunit phospho (Ser1166) antibody

anti-NMDA NR2B Subunit phospho (Ser1166) antibody for Western blot and Rat,Mouse

Neuroscience antibody; Postsynaptic Receptor antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes NMDA NR2B Subunit phospho (Ser1166)
Tested Reactivity Ms, Rat
Predict Reactivity Hu, Dog, NHuPrm, Xenopus laevis
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NMDA NR2B Subunit
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1166 conjugated to KLH
Conjugation Un-conjugated
Alternate Names MRD6; EIEE27; NR2B; hNR3; GluN2B; NR3; N-methyl D-aspartate receptor subtype 2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; NMDAR2B

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:250
Application Note Specific for the ~180k NMDAR NR2B subunit phosphorylated at Ser1166. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14812 Mouse GRIN2B

GeneID: 24410 Rat GRIN2B

Swiss-port # Q00960 Rat Glutamate receptor ionotropic, NMDA 2B

Swiss-port # Q01097 Mouse Glutamate receptor ionotropic, NMDA 2B

Gene Symbol GRIN2B
Gene Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2B
Background The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Phosphorylation of Ser1166 is thought to play an essential role in memory and neuronal development.
Research Area Neuroscience antibody; Postsynaptic Receptor antibody
Calculated MW 166 kDa
PTM Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity.

Images (1) Click the Picture to Zoom In

  • ARG52366 anti-NMDA NR2B Subunit phospho (Ser1166) antibody WB image

    Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser 1166 (Control) stained with ARG52366 anti-NMDA NR2B Subunit phospho (Ser1166) antibody.
    Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide).
    .