anti-NMDAR1 N1 antibody
anti-NMDAR1 N1 antibody for Immunohistochemistry,Western blot and Mouse,Rat
|Product Description||Rabbit Polyclonal antibody recognizes NMDAR1 N1|
|Tested Reactivity||Ms, Rat|
|Predict Reactivity||Hu, Chk, Dog, NHuPrm, Xenopus laevis|
|Tested Application||IHC, WB|
|Target Name||NMDAR1 N1|
|Immunogen||Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert conjugated to KLH|
|Alternate Names||NMDA1; GluN1; MRD8; NMD-R1; Glutamate receptor ionotropic, NMDA 1; Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; NR1; NMDAR1|
|Application Note||Specific for the ~120k NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||glutamate receptor, ionotropic, N-methyl D-aspartate 1|
|Background||The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory and neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.|
|Research Area||Neuroscience antibody|
|Calculated MW||105 kDa|
|PTM||NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.|
Images (1) Click the Picture to Zoom In
ARG52356 anti-NMDAR1 N1 antibody WB image
Western blot: 10 μg of HEK 293 cells expressing:
Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor containing the N1 splice variant insert.
Lane 2 - NR1 subunit containing only the C2 Insert;
Lane 3 - NR1 subunit containing the C1 and C2' Insert;
Lane 4 - without NR1 expression (Mock).
stained with ARG52356 anti-NMDAR1 N1 antibody.