anti-NMDAR2A phospho (Tyr1325) antibody

anti-NMDAR2A phospho (Tyr1325) antibody for Western blot and Mouse,Rat

Neuroscience antibody; Postsynaptic Receptor antibody


Product Description Rabbit Polyclonal antibody recognizes NMDAR2A phospho (Tyr1325)
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NMDAR2A
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 conjugated to KLH
Conjugation Un-conjugated
Alternate Names FESD; NR2A; GluN2A; Glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; EPND; Glutamate [NMDA] receptor subunit epsilon-1; NMDAR2A; LKS; hNR2A

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely blocked by blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 14811 Mouse GRIN2A

GeneID: 24409 Rat GRIN2A

Swiss-port # P35436 Mouse Glutamate receptor ionotropic, NMDA 2A

Swiss-port # Q00959 Rat Glutamate receptor ionotropic, NMDA 2A

Gene Symbol GRIN2A
Gene Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2A
Background The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).
Research Area Neuroscience antibody; Postsynaptic Receptor antibody
Calculated MW 165 kDa

Images (1) Click the Picture to Zoom In

  • ARG52361 anti-NMDAR2A phospho (Tyr1325) antibody WB image

    Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDAR phosphorylated at Tyr1325 (Control) stained with ARG52361 anti-NMDAR2A phospho (Tyr1325) antibody. The phosphospecificity of this labeling is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).