ARG52367

anti-NMDAR2B phospho (Tyr1252) antibody

anti-NMDAR2B phospho (Tyr1252) antibody for Western blot,Immunohistochemistry and Rat

Neuroscience antibody; Postsynaptic Receptor antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes NMDAR2B phospho (Tyr1252)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Bov, Chk, Dog, NHuPrm, Zfsh
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NMDAR2B
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 conjugated to KLH
Conjugation Un-conjugated
Alternate Names MRD6; EIEE27; NR2B; hNR3; GluN2B; NR3; N-methyl D-aspartate receptor subtype 2B; Glutamate receptor ionotropic, NMDA 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; NMDAR2B

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:400
WB1:1000
Application Note Specific for ~180k NMDAR NR2B subunit protein phosphorylated at Tyr1252. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by λ-phosphatase treatment. The antibody may also show some slight reactivity with Tyr1246 of NR2A.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24410 Rat GRIN2B

Swiss-port # Q00960 Rat Glutamate receptor ionotropic, NMDA 2B

Gene Symbol GRIN2B
Gene Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 2B
Background The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1252 is thought to potentiate NMDA receptordependent influx of calcium (Takasu et al., 2002).
Research Area Neuroscience antibody; Postsynaptic Receptor antibody
Calculated MW 166 kDa
PTM Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity.

Images (2) Click the Picture to Zoom In

  • ARG52367 anti-NMDAR2B phospho (Tyr1252) antibody WB image

    Western blot: Detection of NMDA NR2B protein in Rat hippocampal lysate (showing secificity, lane 1) and lambda-phosphatase incubated Rat hippocampal lysate (negative control, lane 2) stained with ARG52367 anti-NMDAR2B phospho (Tyr1252) antibody.

  • ARG52367 anti-NMDAR2B phospho (Tyr1252) antibody ICC/IF image

    Immunofluorescence: 14 DIV Rat cortical neurons stained with ARG52367 anti-NMDAR2B phospho (Tyr1252) antibody showing NR2B phosphorylated at Tyr 1252 in red and PSD95 in green.