anti-NOX2 / gp91phox antibody

anti-NOX2 / gp91phox antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Immune System antibody; Metabolism antibody
publication_link Publication2


Product Description Rabbit Polyclonal antibody recognizes NOX2 / gp91phox
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NOX2 / gp91phox
Antigen Species Human
Immunogen Recombinant protein of Human CYBB (NP_000388.2)
Conjugation Un-conjugated
Alternate Names Neutrophil cytochrome b 91 kDa polypeptide; gp91-phox; NADPH oxidase 2; Heme-binding membrane glycoprotein gp91phox; 558; CGD91-phox; gp91-1; EC 1.-.-.-; Cytochrome b; GP91-1; IMD34; CGD; GP91-PHOX; AMCBX2; GP91PHOX; NOX2; Cytochrome b-245 heavy chain; p22 phagocyte B-cytochrome; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit; Cytochrome b558 subunit beta

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control THP-1


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 13058 Mouse CYBB

GeneID: 1536 Human CYBB

Swiss-port # P04839 Human Cytochrome b-245 heavy chain

Swiss-port # Q61093 Mouse Cytochrome b-245 heavy chain

Gene Symbol CYBB
Gene Full Name cytochrome b-245, beta polypeptide
Background Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
Function Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. [UniProt]
Research Area Cancer antibody; Immune System antibody; Metabolism antibody
Calculated MW 65 kDa
PTM Glycosylated.
Phosphorylated on Ser and Thr residues.

Images (3) Click the Picture to Zoom In

  • ARG55310 anti-NOX2 / gp91phox antibody ICC/IF image

    Immunofluorescence: A431 cells stained with ARG55310 anti-NOX2 / gp91phox antibody at 1:100 dilution.

  • ARG55310 anti-NOX2 / gp91phox antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG55310 anti-NOX2 / gp91phox antibody at 1:100 dilution.

  • ARG55310 anti-NOX2 / gp91phox antibody WB image

    Western blot: THP-1 cell lysate stained with ARG55310 anti-NOX2 / gp91phox antibody.

Specific References

Pterostilbene Interferes With Lipopolysaccharide-Induced Myocardial Injury Through Oxidative Stress and Inflammasome Pathways

WB / Mouse

Lei Zhang et al.
Front Physiol .,  (2022)




Neohesperidin Protects Angiotensin II-Induced Hypertension and Vascular Remodeling.

WB / Mouse

Zhang J et al.
Frontiers in Pharmacology,  (2022)