anti-NOX4 antibody

anti-NOX4 antibody for ELISA,Immunohistochemistry,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
publication_link Publication3


Product Description

Rabbit Polyclonal antibody recognizes NOX4

Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, IHC, WB
Specificity NOX4 antibody is human, mouse, and rat reactive. At least four isoforms of NOX4 are known to exist. NOX4 is predicted to not cross-react with other NOX proteins.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NOX4
Antigen Species Human
Immunogen Synthetic peptide (14 aa) within aa. 70-120 of Human NOX4.
Conjugation Un-conjugated
Alternate Names RENOX; KOX-1; P; Renal NAD; Kidney oxidase-1; NADPH oxidase 4; KOX; EC 1.6.3.-; Kidney superoxide-producing NADPH oxidase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC5 - 20 μg/ml
WB1 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat Cell Lysate


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 50490 Mouse NOX4

GeneID: 50507 Human NOX4

GeneID: 85431 Rat NOX4

Gene Symbol NOX4
Gene Full Name NADPH oxidase 4
Background The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide. NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active. The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.
Function Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.
Isoform 4: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1. [UniProt]
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Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 67 kDa
PTM Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.

Images (3) Click the Picture to Zoom In

  • ARG55254 anti-NOX4 antibody WB image

    Western blot: 30 µg of Jurkat cell lysate stained with ARG55254 anti-NOX4 antibody at 1:500 dilution.

  • ARG55254 anti-NOX4 antibody IHC image

    Immunohistochemistry: NOX4 in human spleen tissue stained with ARG55254 anti-NOX4 antibody at 20 μg/ml dilution.

  • ARG55254 anti-NOX4 antibody IHC image

    Immunohistochemistry: NOX4 in human spleen tissue stained with ARG55254 anti-NOX4 antibody at 5 μg/ml dilution.

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Sample:293T and Jurkat

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Pterostilbene Interferes With Lipopolysaccharide-Induced Myocardial Injury Through Oxidative Stress and Inflammasome Pathways

WB / Mouse

Lei Zhang et al.
Front Physiol .,  (2022)




Neohesperidin Protects Angiotensin II-Induced Hypertension and Vascular Remodeling.

WB / Mouse

Zhang J et al.
Frontiers in Pharmacology,  (2022)




Treatment effects of the traditional Chinese medicine Shenks in bleomycin-induced lung fibrosis through regulation of TGF-beta/Smad3 signaling and oxidative stress.

IHC-P / Mouse

Haiyan Chu et al.
Sci Rep. ,  (2017)