anti-NSE / Neuron Specific Enolase antibody [5A4]

anti-NSE / Neuron Specific Enolase antibody [5A4] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody


Product Description Mouse Monoclonal antibody [5A4] recognizes NSE / Neuron Specific Enolase
Tested Reactivity Hu
Tested Application ELISA, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 5A4
Isotype IgG2a
Target Name NSE / Neuron Specific Enolase
Antigen Species Human
Immunogen Purified NSE from human brain.
Conjugation Un-conjugated
Alternate Names Neural enolase; NSE; Enolase 2; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; HEL-S-279; Neuron-specific enolase; EC

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein G affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 2026 Human ENO2

Swiss-port # P09104 Human Gamma-enolase

Gene Symbol ENO2
Gene Full Name enolase 2 (gamma, neuronal)
Background This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). [UniProt]
Highlight Related products:
NSE antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Neuronal Development Marker
Research Area Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 47 kDa

Clone References

Evidence that a panel of neurodegeneration biomarkers predicts vasospasm, infarction, and outcome in aneurysmal subarachnoid hemorrhage.

WB / Human

Siman R et al.
PLoS One.,  (2011)




Ionizing radiation induces prostate cancer neuroendocrine differentiation through interplay of CREB and ATF2: implications for disease progression.

WB / Human

Deng X et al.
Cancer Res.,  (2008)