ARG45494

anti-NSUN6 antibody

anti-NSUN6 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes NSUN6
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name NSUN6
Antigen Species Human
Immunogen Recombinant protein containing to human NSUN6.
Conjugation Un-conjugated
Alternate Names NSUN6; NOP2/Sun RNA Methyltransferase 6; NOPD1; NOL1/NOP2/Sun And PUA Domain-Containing Protein 1; TRNA (Cytosine(72)-C(5))-Methyltransferase NSUN6; NOL1/NOP2/Sun Domain Family, Member 6; NOP2/Sun Domain Family, Member 6; ARL5B Antisense RNA 1; ARL5B-AS1; FLJ23743; Nucleolar Protein (NOL1/NOP2/Sun) And PUA Domains 1; NOP2/Sun RNA Methyltransferase Family Member 6; NOL1/NOP2/Sun Domain Family Member 6; Putative Methyltransferase NSUN6; 4933414E04Rik; EC 2.1.1.52 47; EC 2.1.1.-; EC 2.1.1 47; MRT82

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 52 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 221078 Human NSUN6

GeneID: 74455 Mouse NSUN6

Swiss-port # Q7TS68 Mouse Putative methyltransferase NSUN6

Swiss-port # Q8TEA1 Human Putative methyltransferase NSUN6

Gene Symbol NSUN6
Gene Full Name NOP2/Sun RNA Methyltransferase 6
Background Enables tRNA (cytidine-5-)-methyltransferase activity and tRNA binding activity. Involved in tRNA C5-cytosine methylation. Located in cytoplasm. Implicated in autosomal recessive intellectual developmental disorder 82. [provided by Alliance of Genome Resources, Feb 2025]
Function S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 52 kDa
PTM Acetylation. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45494 anti-NSUN6 antibody IHC-P image

    Immunohistochemistry: Human liver cancer stained with ARG45494 anti-NSUN6 antibody at 2 μg/ml dilution.

  • ARG45494 anti-NSUN6 antibody WB image

    Western blot: 293T stained with ARG45494 anti-NSUN6 antibody at 0.5 μg/ml dilution.

  • ARG45494 anti-NSUN6 antibody FACS image

    Flow Cytometry: 293T stained with ARG45494 anti-NSUN6 antibody at 1 µg/10^6 cells dilution.

  • ARG45494 anti-NSUN6 antibody WB image

    Western blot: Rat brain stained with ARG45494 anti-NSUN6 antibody at 0.5 μg/ml dilution.

  • ARG45494 anti-NSUN6 antibody WB image

    Western blot: Mouse brain stained with ARG45494 anti-NSUN6 antibody at 0.5 μg/ml dilution.