ARG10813

anti-Natriuretic Peptide Receptor A antibody

anti-Natriuretic Peptide Receptor A antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,Immunohistochemistry,IHC-Whole mount,Immunoprecipitation,Western blot,Dot blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Natriuretic Peptide Receptor A
Tested Reactivity Hu, Ms, Rat
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, IHC-Wmt, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Natriuretic Peptide Receptor A
Antigen Species Human
Immunogen Synthetic peptide around aa. 1042-1057 of Human Natriuretic Peptide Receptor A. (VRTYWLLGERGCSTRG)
Conjugation Un-conjugated
Full Name natriuretic peptide receptor 1
Alternate Names ANPa; GUCY2A; GC-A; ANPRA; Atrial natriuretic peptide receptor 1; Guanylate cyclase A; NPR-A; NPRA; GUC2A; Atrial natriuretic peptide receptor type A; EC 4.6.1.2; ANP-A; ANPR-A

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:25 - 1:250
Dot1:10000
ELISA1:10000
ICC/IF1:25 - 1:250
IHC1:25 - 1:250
IHC-WmtAssay-dependent
IP1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 119 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18160 Mouse NPR1

GeneID: 24603 Rat NPR1

GeneID: 4881 Human NPR1

Gene Symbol NPR1
Gene Full Name natriuretic peptide receptor 1
Background Guanylyl cyclases, catalyzing the production of cGMP from GTP, are classified as soluble and membrane forms (Garbers and Lowe, 1994 [PubMed 7982997]). The membrane guanylyl cyclases, often termed guanylyl cyclases A through F, form a family of cell-surface receptors with a similar topographic structure: an extracellular ligand-binding domain, a single membrane-spanning domain, and an intracellular region that contains a protein kinase-like domain and a cyclase catalytic domain. GC-A and GC-B function as receptors for natriuretic peptides; they are also referred to as atrial natriuretic peptide receptor A (NPR1) and type B (NPR2; MIM 108961). Also see NPR3 (MIM 108962), which encodes a protein with only the ligand-binding transmembrane and 37-amino acid cytoplasmic domains. NPR1 is a membrane-bound guanylate cyclase that serves as the receptor for both atrial and brain natriuretic peptides (ANP (MIM 108780) and BNP (MIM 600295), respectively).[supplied by OMIM, May 2009]
Function Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand. [UniProt]
PTM Phosphorylation of the protein kinase-like domain is required for full activation by ANP.

Images (4) Click the Picture to Zoom In

  • ARG10813 anti-Natriuretic Peptide Receptor A antibody IHC image

    Immunohistochemistry: Thin section of Mouse kidney tissue stained with ARG10813 anti-Natriuretic Peptide Receptor A antibody (red). Blue: Nuclei.

  • ARG10813 anti-Natriuretic Peptide Receptor A antibody WB image

    Western blot: Recombinant Natriuretic Peptide Receptor A protein stained with ARG10813 anti-Natriuretic Peptide Receptor A antibody at 1:500 dilution.

  • ARG10813 anti-Natriuretic Peptide Receptor A antibody IHC image

    Immunohistochemistry: Thin section of Mouse heart tissue stained with ARG10813 anti-Natriuretic Peptide Receptor A antibody (red). Blue: Nuclei.

  • ARG10813 anti-Natriuretic Peptide Receptor A antibody IHC-Wmt image

    Immunohistochemistry: Whole-mount Mouse nose stained with ARG10813 anti-Natriuretic Peptide Receptor A antibody (red). Green: Grueneberg ganglion neurons. Blue: Nuclei.