ARG10731

anti-Nestin antibody

anti-Nestin antibody for ICC/IF,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Nestin
Tested Reactivity Hu
Species Does Not React With Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Nestin
Antigen Species Human
Immunogen Synthetic peptide around aa. 315-630 of Human Nestin.
Conjugation Un-conjugated
Full Name nestin
Alternate Names Nestin; Nbla00170

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:5000
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 177 kDa

Properties

Form Liquid
Purification Unpurified.
Buffer Serum.
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 10763 Human NES

Swiss-port # P48681 Human Nestin

Gene Symbol NES
Gene Full Name nestin
Background This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
Function Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity). [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; NSC antibody; Neural Stem Cell Marker antibody; NSC and Neuron Marker antibody; Neuron Development Study antibody
PTM Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).

Images (4) Click the Picture to Zoom In

  • ARG10731 anti-Nestin antibody ICC/IF image

    Immunocytochemistry: SH-SY5Y neuroblastoma cells were stained with ARG10731 anti-Nestin antibody at 1:5000 (red) and co-stained with an antibody against vimentin at 1:10000 (green).  Vimentin is main partner of nestin forming heterdimers and further polymerizing to form intermediate filaments. Co-localization of vimentin and nestin in cells was shown in yellow. Blue is DAPI staining of DNA.

  • ARG10731 anti-Nestin antibody ICC/IF image

    Immunofluorescence: SH-SY5Y cells stained with ARG10731 anti-Nestin antibody (green) at 1:3000 dilution and costained with Mouse mAb to actin (red) at 1:500 dilution. Hoechst (blue) for nuclear staining.

    The Nestin antibody produces strong staining in the cytoplasm in clearly filamentous fashion, while actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

  • ARG10731 anti-Nestin antibody WB image

    Western blot: SH-SY5Y homogenate stained with ARG10731 anti-Nestin antibody at 1:2000 dilution.

  • ARG10731 anti-Nestin antibody WB image

    Western blot: Rat cortical neuron-glial cell and SH-SY5Y cell lysates stained with ARG10731 anti-Nestin antibody (green) at 1:3000 dilution.

    The blot detected only in Human cells.