ARG52348

anti-Neurofilament NF-L antibody [DA2]

anti-Neurofilament NF-L antibody [DA2] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody
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Overview

Product Description

Mouse Monoclonal antibody [DA2] recognizes Neurofilament NF-L

Tested Reactivity Hu, Ms, Rat
Predict Reactivity Chk
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone DA2
Isotype IgG1
Target Name Neurofilament NF-L
Antigen Species Pig
Immunogen Preparation of porcine NF-L
Epitope Epitope is likely centered on the HVQEEQIEVE peptide.
Conjugation Un-conjugated
Alternate Names Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IF1:100 - 1:1000
IHC-Fr1:100 - 1:1000
IHC-PAssay-dependent
WB1:1000 - 1:5000
Application Note Specific for the ~68k Neurofilament L protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Total IgG fraction
Buffer Total IgG fraction and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18039 Mouse NEFL

GeneID: 4747 Human NEFL

GeneID: 83613 Rat NEFL

Background Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H . NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease .
Highlight Related Antibody Duos and Panels:
ARG30010 Neuronal Cytoskeletal Antibody Duo (NF-L, TUBBIII)
ARG30011 Neurofilament Antibody Duo (NF-H, NF-L)
ARG30145 Intermediate Neurofilament Antibody Panel (NF-L, NF-M,NF-H)
Related products:
Neurofilament antibodies; Neurofilament ELISA Kits; Neurofilament Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody
Calculated MW 62 kDa
PTM O-glycosylated.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Ubiquitinated in the presence of TRIM2 and UBE2D1.

Images (4) Click the Picture to Zoom In

  • ARG52348 anti-Neurofilament NF-L antibody [DA2] WB image

    Western blot: 30 µg of Mouse brain lysate stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] at 1:1000 dilution.

  • ARG52348 anti-Neurofilament NF-L antibody [DA2] WB image

    Western blot: rat cortex lysate stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] showing specific immunolableing of the ~ 68k NF-L protein.

  • ARG52348 anti-Neurofilament NF-L antibody [DA2] IHC-Fr image

    Immunohistochemistry: Frozen section of Rat frontal cortex tissue stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] (red) at 1:500 dilution, and costained with anti-GFAP antibody (green) at 1:5000 dilution. Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.

    Clone DA2 labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells, while the GFAP antibody stains the network of glial cells.

  • ARG52348 anti-Neurofilament NF-L antibody [DA2] WB image

    Western blot: Rat brain, Rat spinal cord, Mouse brain and Mouse spinal cord lysates stained with ARG52348 anti-Neurofilament NF-L antibody [DA2] (green) at 1:5000 dilution.

Customer's Feedback

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Review for anti-Neurofilament NF-L antibody [DA2]

Application:WB

Sample:Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells.

WB / Human

Shaw G et al.
FASEB J.,  (2002)

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Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.

WB / Rat

Benson DL et al.
J Neurocytol.,  (1996)

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Identification of a tektin-like protein associated with neurofilaments in the developing chick nervous system.

ICC/IF / Chicken

Edson KJ et al.
J Neurosci Res.,  (1991)

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