ARG22289

anti-Neuroligin 3 antibody [S110-29]

anti-Neuroligin 3 antibody [S110-29] for Western blot,Immunohistochemistry,ICC/IF and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [S110-29] recognizes Neuroligin 3
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC, WB
Specificity Detects ~110kDa. Does not cross-react with Neuroligin-1, -2, -4 or -4.
Host Mouse
Clonality Monoclonal
Clone S110-29
Isotype IgG1
Target Name Neuroligin 3
Antigen Species Rat
Immunogen Fusion protein around aa. 730-848 (intracellular C-terminus) of Rat Neuroligin 3.
Mouse: 99% identity (118/119 around aa. identical).
Human: 98% identity (116/119 around aa. identical); ~60% identity with Neuroligin 1; ~40% identity with Neuroligin-2.
Conjugation Un-conjugated
Alternate Names Gliotactin homolog; Neuroligin-3; HNL3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHCAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol
Preservative 0.1% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 171297 Rat NLGN3

GeneID: 245537 Mouse NLGN3

GeneID: 54413 Human NLGN3

Gene Symbol Nlgn3
Gene Full Name neuroligin 3
Background This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. Mutations in this gene may be associated with autism and Asperger syndrome. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Oct 2009]
Function Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and may mediate its effects by clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system (By similarity). [UniProt]
Cellular Localization Cell Junction, Cell membrane, Synapse
Calculated MW 94 kDa

Images (2) Click the Picture to Zoom In

  • ARG22289 anti-Neuroligin 3 antibody [S110-29] ICC/IF image

    Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22289 anti-Neuroligin 3 antibody [S110-29] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22289 anti-Neuroligin 3 antibody [S110-29] (D) Composite.

  • ARG22289 anti-Neuroligin 3 antibody [S110-29] WB image

    Western blot: 15 µg of Mouse Brain Membrane. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: ARG22289 anti-Neuroligin 3 antibody [S110-29] at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT.