ARG22269

anti-Nitrotyrosine antibody [39B6]

anti-Nitrotyrosine antibody [39B6] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation,ELISA,Flow cytometry and Other

Overview

Product Description Mouse Monoclonal antibody [39B6] recognizes Nitrotyrosine
Tested Reactivity Other
Tested Application ELISA, FACS, ICC/IF, IHC, IP, WB
Specificity Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
Host Mouse
Clonality Monoclonal
Clone 39B6
Isotype IgG2a
Target Name Nitrotyrosine
Immunogen Hybridoma line 39B6
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC1:100
IPAssay-dependent
WB1:1400
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS, 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG22269 anti-Nitrotyrosine antibody [39B6] IHC image

    Immunohistochemistry: Rat liver tissue stained with ARG22269 anti-Nitrotyrosine antibody [39B6] at 1:1000 dilution.

  • ARG22269 anti-Nitrotyrosine antibody [39B6] IHC image

    Immunohistochemistry: Formalin fixed inflamed colon stained with ARG22269 anti-Nitrotyrosine antibody [39B6] at 1:1000000 dilution (12 hours). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200uL for 2 min at RT. Magnification: 40x.

  • ARG22269 anti-Nitrotyrosine antibody [39B6] IHC image

    Immunohistochemistry: Formalin fixed colon carcinoma stained with ARG22269 anti-Nitrotyrosine antibody [39B6] at 1:25000 dilution (12 hours). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200uL for 2 min at RT. Magnification: 40x.