ARG56179

anti-Nuclear antigen antibody [235-1]

anti-Nuclear antigen antibody [235-1] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human

Overview

Product Description Mouse Monoclonal antibody [235-1] recognizes Nuclear antigen
Tested Reactivity Hu
Species Does Not React With Ms, Rat, Chk
Tested Application FACS, ICC/IF, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone 235-1
Isotype IgG1, kappa
Target Name Nuclear antigen
Antigen Species Human
Immunogen Nuclei of human myeloid leukemia biopsy cells.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-Fr1 - 2 µg/ml
IP1 - 2 µg/500 µg lysate
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Cellular Localization Nuclear

Images (11) Click the Picture to Zoom In

  • ARG56179 anti-Nuclear antigen antibody [235-1] ICC/IF image

    Immunofluorescence: Paraformaldehyde-fixed MCF7 cells stained with ARG56179 anti-Nuclear antigen antibody [235-1] (green) and counterstained with Phalloidin (red).

  • ARG56179 anti-Nuclear antigen antibody [235-1] FACS image

    Flow Cytometry: Testing of MCF-7 cells. Black: No antibodies; Green: Isotype control; Red: PE-conjugated ARG56179 anti-Nuclear antigen antibody [235-1].

  • ARG56179 anti-Nuclear antigen antibody [235-1] IHC-Fr image

    Immunohistochemistry: Acetone-fixed frozen section of Human tonsil tissue stained with ARG56179 anti-Nuclear antigen antibody [235-1].

  • ARG56179 anti-Nuclear antigen antibody [235-1] FACS image

    Flow Cytometry: PFA-fixed MCF7 cells stained with ARG56179 anti-Nuclear antigen antibody [235-1] (blue); Isotype control (red).

  • ARG56179 anti-Nuclear antigen antibody [235-1] ICC/IF image

    Immunofluorescence: Paraformaldehyde-fixed K562 cells stained with ARG56179 anti-Nuclear antigen antibody [235-1] (green) and counterstained with Phalloidin (red).

  • ARG56179 anti-Nuclear antigen antibody [235-1] ICC/IF image

    Immunofluorescence: Paraformaldehyde-fixed HeLa cells stained with ARG56179 anti-Nuclear antigen antibody [235-1] (green) and counterstained with Phalloidin (red).

  • ARG56179 anti-Nuclear antigen antibody [235-1] FACS image

    Flow Cytometry: Testing of HeLa cells. Black: No antibodies; Green: Isotype control; Red: PE-conjugated ARG56179 anti-Nuclear antigen antibody [235-1].

  • ARG56179 anti-Nuclear antigen antibody [235-1] FACS image

    Flow Cytometry: PFA-fixed HeLa cells stained with ARG56179 anti-Nuclear antigen antibody [235-1] (blue); Isotype control (red).

  • ARG56179 anti-Nuclear antigen antibody [235-1] FACS image

    Flow Cytometry: PFA-fixed Jurkat cells stained with ARG56179 anti-Nuclear antigen antibody [235-1] (blue); Isotype control (red).

  • ARG56179 anti-Nuclear antigen antibody [235-1] FACS image

    Flow Cytometry: PFA-fixed K562 cells stained with ARG56179 anti-Nuclear antigen antibody [235-1] (blue); Isotype control (red).

  • ARG56179 anti-Nuclear antigen antibody [235-1] FACS image

    Flow Cytometry: PFA-fixed Raji cells stained with ARG56179 anti-Nuclear antigen antibody [235-1] (blue); Isotype control (red).